Product Pathways - Metabolism
SignalSilence® Acetyl-CoA Carboxylase 1 siRNA I (Mouse Specific) #6397
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Western blot analysis of extracts from NIH/3T3 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® Acetyl-CoA Carboxylase 1 siRNA I (Mouse Specific) (+) or SignalSilence® Acetyl-CoA Carboxylase 1 siRNA II (Mouse Specific) #6398 (+), using Acetyl-CoA Carboxylase 1 Antibody #4190 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Acetyl-CoA Carboxylase 1 Antibody confirms silencing of Acetyl-CoA Carboxylase 1 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.
SignalSilence® Acetyl-CoA Carboxylase 1 siRNA I (Mouse Specific) from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Acetyl-CoA Carboxylase 1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
CST recommends transfection with 100 nM SignalSilence® Acetyl-CoA Carboxylase 1 siRNA I (Mouse Specific) 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.
Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.
Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA (1). It is the key enzyme in the biosynthesis and oxidation of fatty acids (1). In rodents, the 265 kDa ACC1 (ACCα) form is primarily expressed in lipogenic tissues, while 280 kDa ACC2 (ACCβ) is the main isoform in oxidative tissues (1,2). However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues (1,2). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). ACC is a potential target of anti-obesity drugs (4,5).
- Castle, J.C. et al. (2009) PLoS One 4, e4369.
- Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86.
- Ha, J. et al. (1994) J Biol Chem 269, 22162-8.
- Abu-Elheiga, L. et al. (2001) Science 291, 2613-6.
- Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50.
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- 6568 SignalSilence® Control siRNA (Unconjugated)
- 6201 SignalSilence® Control siRNA (Fluorescein Conjugate)
- 6398 SignalSilence® Acetyl-CoA Carboxylase 1 siRNA II (Mouse Specific)
- 3676 Acetyl-CoA Carboxylase (C83B10) Rabbit mAb
Limited Use Label License, RNA interference: This product is licensed under European Patent 1144623 and foreign equivalents from Ribopharma AG, Kulmbach, Germany and is provided only for use in non-commercial research specifically excluding use (a) in drug discovery or drug development, including target identification or target validation, by or on behalf of a commercial entity, (b) for contract research or commercial screening services, (c) for the production or manufacture of siRNA-related products for sale, or (d) for the generation of commercial databases for sale to Third Parties. Information about licenses for these and other commercial uses is available from Ribopharma AG, Fritz-Hornschuch-Str. 9, D-95326 Kulmbach, Germany.
For Research Use Only. Not For Use In Diagnostic Procedures.