Cell Signaling Technology

Product Pathways - Vesicle Trafficking

REPS1 (D6F4) Rabbit mAb #6404

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 125 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

REPS1 (D6F4) Rabbit mAb recognizes endogenous levels of total REPS1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn189 of human REPS1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using REPS1 (D6F4) Rabbit mAb.

Background

REPS1 is a RalBP1-associated EH-homology domain containing protein. The sequence of REPS1 has an EH domain, followed by two proline-rich segments, and a C-terminal coiled-coil domain for binding to RalBP1 (1). The EH domain of REPS1 interacts with the NPF motif of Rab11-FIP2, mediates their colocalization to endosome vesicles, and influences EGFR endocytosis (2). The two proline-rich regions of REPS1 are important for binding to the SH3 domain of GRK/GRB2 and further regulate EGFR downstream signaling. The proline-rich regions of REPS1 have also been shown to interact with the SH3 domain of intersectin1 (ITSN1) and contribute to ITSN1/SGIP1/REPS1 complex formation on clathrin-coated pits (3). Three alternatively spliced isoforms of REPS1 have been identified.

  1. Yamaguchi, A. et al. (1997) J Biol Chem 272, 31230-4.
  2. Cullis, D.N. et al. (2002) J Biol Chem 277, 49158-66.
  3. Dergai, O. et al. (2010) Biochem Biophys Res Commun 402, 408-13.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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