Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

DR1 Antibody #6447

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk (Dm) (Z) (Dg) (Pg) Endogenous 19 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Dm=D. melanogaster  Z=Zebrafish  Dg=Dog  Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

DR1 Antibody recognizes endogenous levels of total DR1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly112 of human DR1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using DR1 Antibody.

Background

Down-regulator of transcription 1 (DR1), also known as negative cofactor 2-β (NC2-β), forms a heterodimer with DR1 associated protein 1 (DRAP1)/NC2-α and acts as a negative regulator of RNA polymerase II and III (RNAPII and III) transcription (1-5). DR1 activity is thought to be important for modulating the switch between basal transcription activity and transcription activator driven transcription (2,6,7). DR1 interaction with TATA binding protein (TBP) blocks the association of general transcription factors TFIIA and TFIIB with TBP and disrupts the formation of the RNAPII transcription initiation complex (1,8,9). RNAPIII driven transcription is also inhibited by DR1 interaction with TBP. DR1 disrupts the interaction of TBP with the TFIIB related factor (BRF)/RNAPIII B-related factor, inhibiting transcription initiation by the RNAPIII machinery (4).

  1. Inostroza, J.A. et al. (1992) Cell 70, 477-89.
  2. Meisterernst, M. and Roeder, R.G. (1991) Cell 67, 557-67.
  3. Mermelstein, F. et al. (1996) Genes Dev 10, 1033-48.
  4. White, R.J. et al. (1994) Science 266, 448-50.
  5. Kantidakis, T. and White, R.J. (2010) Nucleic Acids Res 38, 1228-39.
  6. Kraus, V.B. et al. (1994) Proc Natl Acad Sci U S A 91, 6279-82.
  7. Yeung, K.C. et al. (1994) Genes Dev 8, 2097-109.
  8. Kim, T.K. et al. (1995) J Biol Chem 270, 10976-81.
  9. Kamada, K. et al. (2001) Cell 106, 71-81.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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