Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Cytoskeletal Signaling

α-Actinin (D6F6) XP® Rabbit mAb #6487

Applications Reactivity Sensitivity MW (kDa) Isotype
W IF-IC H M R Mk (Z) Endogenous 100 Rabbit IgG

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Z=Zebrafish
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

* Product-specific protocol.

Specificity / Sensitivity

α-Actinin (D6F6) XP® Rabbit mAb recognizes endogenous levels of total α-actinin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe316 of human α-actinin protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using α-Actinin (D6F6) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of SNB19 cells using α-Actinin (D6F6) XP® Rabbit mAb (green) showing colocalization with actin filaments that were labeled with β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

α-Actinin belongs to the spectrin family of cytoskeletal proteins. It was first recognized as an actin cross-linking protein, forming an antiparallel homodimer with an actin binding head at the amino terminus of each monomer. More recently, α-actinin has been shown to interact with a large number of proteins involved in signaling to the cytoskeleton including those involved in cellular adhesion, migration, and immune cell targeting (1). The interaction of α-actinin with intercellular adhesion molecule-5 (ICAM-5) helps to promote neurite outgrowth (2). In osteoblasts, interaction of α-actinin with integrins stabilizes focal adhesions and may protect cells from apoptosis (3). Isoforms 1 and 4 of α-actinin, which are non-muscle isoforms, are present in stress fibers, sites of adhesion and intercellular contacts, filopodia, and lamellipodia. The muscle isoforms 2 and 3 localize to the Z-discs of striated muscle and to dense bodies and plaques in smooth muscle (1).

  1. Otey, C.A. and Carpen, O. (2004) Cell Motil. Cytoskeleton 58, 104-111.
  2. Nyman-Huttunen, H. et al. (2006) J. Cell Sci. 119, 3057-3066.
  3. Triplett, J.W. and Pavalko, F.M. (2006) Am. J. Physiol. Cell Physiol. 291, C909-921.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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