Product Pathways - Cytoskeletal Signaling
α-Actinin (D6F6) XP® Rabbit mAb #6487
|W IF-IC||H M R Mk (Z)||Endogenous||100||Rabbit IgG|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Z=Zebrafish
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
* Product-specific protocol.
Specificity / Sensitivity
α-Actinin (D6F6) XP® Rabbit mAb recognizes endogenous levels of total α-actinin protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe316 of human α-actinin protein.
Western blot analysis of extracts from various cell lines using α-Actinin (D6F6) XP® Rabbit mAb.
α-Actinin belongs to the spectrin family of cytoskeletal proteins. It was first recognized as an actin cross-linking protein, forming an antiparallel homodimer with an actin binding head at the amino terminus of each monomer. More recently, α-actinin has been shown to interact with a large number of proteins involved in signaling to the cytoskeleton including those involved in cellular adhesion, migration, and immune cell targeting (1). The interaction of α-actinin with intercellular adhesion molecule-5 (ICAM-5) helps to promote neurite outgrowth (2). In osteoblasts, interaction of α-actinin with integrins stabilizes focal adhesions and may protect cells from apoptosis (3). Isoforms 1 and 4 of α-actinin, which are non-muscle isoforms, are present in stress fibers, sites of adhesion and intercellular contacts, filopodia, and lamellipodia. The muscle isoforms 2 and 3 localize to the Z-discs of striated muscle and to dense bodies and plaques in smooth muscle (1).
- Otey, C.A. and Carpen, O. (2004) Cell Motil. Cytoskeleton 58, 104-111.
- Nyman-Huttunen, H. et al. (2006) J. Cell Sci. 119, 3057-3066.
- Triplett, J.W. and Pavalko, F.M. (2006) Am. J. Physiol. Cell Physiol. 291, C909-921.
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For Research Use Only. Not For Use In Diagnostic Procedures.