Cell Signaling Technology

Product Pathways - Adhesion

Afadin Antibody #6492

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 205 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Afadin Antibody recognizes endogenous levels of total afadin protein. Based on sequence homology, the antibody is expected to recognize all isoforms of afadin.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro574 of human afadin protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Afadin Antibody.

Background

In multicellular organisms, intercellular junctions play essential roles in tissue integrity and maintenance of cell polarity. Tight junctions (TJs) form a continuous barrier to fluids across the epithelium and endothelium (reviewed in 1). Adherens junctions (AJs) are dynamic structures that form cell-cell contacts linking cells into a continuous sheet (reviewed in 2). The actin filament-binding protein, Afadin, binds to nectin forming a connection to the actin cytoskeleton (3). AJs are formed when nectin assembles cadherin at the cell-cell adhesion site and these junctions are then involved in the formation and maintenance of TJs (4,5). Afadin has two splice variants: l-afadin, which is ubiquitously expressed, and s-afadin, which is expressed predominantly in neural tissue. s-Afadin is a shorter form lacking one of the three proline-rich regions found in l-afadin, as well as the carboxyl-terminal F-actin binding region (6). Human s-afadin is identical to AF-6, the ALL-1 fusion partner involved in acute myeloid leukemias (7). Recent work has also shown that afadin is involved in controlling the directionality of cell movement when it is localized at the leading edge of moving cells (8,9).

  1. Shin, K. et al. (2006) Annu Rev Cell Dev Biol 22, 207-35.
  2. Harris, T.J. and Tepass, U. (2010) Nat Rev Mol Cell Biol 11, 502-14.
  3. Ikeda, W. et al. (1999) J Cell Biol 146, 1117-32.
  4. Sato, T. et al. (2006) J Biol Chem 281, 5288-99.
  5. Ooshio, T. et al. (2007) J Cell Sci 120, 2352-65.
  6. Mandai, K. et al. (1997) J Cell Biol 139, 517-28.
  7. Prasad, R. et al. (1993) Cancer Res 53, 5624-8.
  8. Miyata, M. et al. (2009) J Cell Sci 122, 4319-29.
  9. Miyata, M. et al. (2009) J Biol Chem 284, 24595-609.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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