Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Metabolism

ACO2 (D6D9) XP® Rabbit mAb #6571

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H M R Hm Mk Endogenous 85 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ACO2 (D6D9) XP® Rabbit mAb recognizes endogenous levels of total ACO2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly540 of human ACO2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using ACO2 (D6D9) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using ACO2 (D6D9) XP® Rabbit mAb (green), showing colocalization with mitochondria that were labeled with MitoTracker® Red CMXRos (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Aconitase 2 (ACO2) catalyzes the conversion of citrate to isocitrate via cis-aconitate in the second step of the tricarboxylic acid (TCA) cycle (1,2). ACO2 is also an important regulator of iron homeostasis within cells (1-4). In addition, research studies have shown that this enzyme is deficient in the mitochondrial disease Friedreich's Ataxia (4,5).

  1. Gille, G. and Reichmann, H. (2011) J Neural Transm 118, 349-59.
  2. Mirel, D.B. et al. (1998) Gene 213, 205-18.
  3. Myers, C.R. et al. (2010) Free Radic Biol Med 49, 1903-15.
  4. Rötig, A. et al. (1997) Nat Genet 17, 215-7.
  5. Ye, H. and Rouault, T.A. (2010) Biochemistry 49, 4945-56.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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