Cell Signaling Technology

Product Pathways - Apoptosis

PARP (46D11) Rabbit mAb (Sepharose Bead Conjugate) #6704

Applications Reactivity Sensitivity MW (kDa) Isotype
IP H M R Mk Endogenous 116, 89 Rabbit IgG

Applications Key:  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PARP (46D11) Rabbit mAb (Sepharose Bead Conjugate) detects endogenous levels of total full-length PARP and the large fragment (89 kDa) produced by caspase cleavage.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly623 of PARP protein.

IP

IP

Immunoprecipitation of THP-1 cell lysates, untreated or cycloheximide and TNFα-treated, using PARP (46D11) Rabbit mAb (Sepharose Bead Conjugate). The blot was probed using PARP (46D11) Rabbit mAb #9532.

IP

IP

Immunoprecipitation of HeLa cell lysates, untreated or staurosporine-treated, using PARP (46D11) Rabbit mAb (Sepharose Bead Conjugate). The blot was probed using PARP (46D11) Rabbit mAb #9532.

Description

This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. PARP (46D11) Rabbit mAb (Sepharose Bead Conjugate) is useful for immunoprecipitation assays. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated PARP (46D11) Rabbit mAb #9532.

Background

PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).

  1. Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358.
  2. Lazebnik, Y. A. et al. (1994) Nature 371, 346-347.
  3. Cohen, G.M. (1997) Biochem. J. 326, 1-16.
  4. Nicholson, D. W. et al. (1995) Nature 376, 37-43.
  5. Tewari, M. et al. (1995) Cell 81, 801-809.
  6. Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539.

Application References

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Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.

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