Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

RBAP46 (V415) Antibody #6882

Applications Reactivity Sensitivity MW (kDa) Source
W IP IF-IC H M R Mk (Pg) (Hr) Endogenous 48 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Pg=Pig  Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

RBAP46 (V415) Antibody recognizes endogenous levels of total RBAP46 protein. This antibody does not cross-react with the RBAP48 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human RBAP46 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using RBAP46 (V415) Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using RBAP46 (V415) Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red).

Background

Retinoblastoma-associated proteins 46 and 48 (RBAP46 and RBAP48; also known as RBBP7 and RBBP4) were first characterized in human cells as proteins that bind to the retinoblastoma (Rb) tumor suppressor protein (1). Since then, these proteins have been shown to be components of many protein complexes involved in chromatin regulation, including the chromatin assembly factor 1 (CAF-1) complex and type B histone acetyltransferase complex HAT1, both of which function in chromatin assembly during DNA replication (2,3). RBAP46 and RBAP48 are also found in the nucleosome remodeling factor complex NURF, the nucleosome remodeling and histone de-acetylation complex NuRD, and the Sin3/HDAC histone de-acetylation complex (4-7). More recently, RBAP46 and RBAP48 were identified as components of the polycomb repressor complex PRC2, which also contains EED and Ezh2 (8). RBAP46 and RBAP48 bind to the histone fold region of histone H4 and are believed to target these chromatin remodeling, histone acetylation, and histone de-acetylation complexes to their histone substrates (3).

  1. Qian, Y.W. et al. (1993) Nature 364, 648-52.
  2. Verreault, A. et al. (1996) Cell 87, 95-104.
  3. Verreault, A. et al. (1998) Curr Biol 8, 96-108.
  4. Barak, O. et al. (2003) EMBO J 22, 6089-100.
  5. Zhang, Y. et al. (1999) Genes Dev 13, 1924-35.
  6. Zhang, Y. et al. (1997) Cell 89, 357-64.
  7. Kuzmichev, A. et al. (2002) Genes Dev 16, 2893-905.
  8. Cao, R. et al. (2002) Science 298, 1039-43.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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