Product Pathways - Chromatin Regulation / Epigenetics
Menin (D45B1) XP® Rabbit mAb #6891
|W IF-IC||H M R Mk (B) (Pg) (Hr)||Endogenous||68||Rabbit IgG|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey B=Bovine Pg=Pig Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Menin (D45B1) XP® Rabbit mAb recognizes total endogenous levels of all 3 isoforms of Menin protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val597 of human Menin protein.
Western blot analysis of extracts from various cell lines using Menin (D45B1) XP® Rabbit mAb.
Mutations in the MEN1 tumor suppressor gene cause multiple endocrine neoplasia type 1 (MEN1), an autosomal dominant familial tumor syndrome typified by tumors of the pituitary, parathyroid, lung, and enteropancreatic endocrine tissues (1,2). Patients with this tumor syndrome have inherited either missense or truncation mutations in one allele of the MEN1 gene, while the other allele is subject to loss of heterozygosity in tumors from these patients (1,2). Menin, the protein product of the MEN1 gene, is a component of the mixed-lineage leukemia protein (MLL)-containing histone methyltransferase complex that facilitates methylation of histone H3 Lys4 to promote transcriptional activation (3,4). Menin functions to suppress proliferation of pancreatic islet cells, at least in part through MLL-mediated activation of the p18INK4c (p18) and p27CIP/KIP (p27) cyclin-dependent kinase inhibitor genes (5,6). Loss of Menin leads to a decrease in methylation of histone H3 Lys4 and decreased expression of the p18 and p27 genes, leading to hyperplasia (5,6). In contrast to its role as a tumor suppressor in endocrine cells, Menin has been shown to promote proliferation in leukemia cells driven by MLL-fusion proteins. Menin is essential for oncogenic MLL-fusion-protein-mediated transformation of bone marrow cells and is required for histone H3 Lys4 methylation and expression of the Hoxa9 gene (7,8). Menin interacts with a wide range of proteins, including JunD, SMAD family members, estrogen receptor, vitamin D receptor, PEM, NFκB, FANCD2, RPA2, NMMHC II-A, GFAP, vimentin, and Hsp70 suggesting additional roles in transcriptional regulation, DNA processing and repair, cytoskeleton organization, and protein degradation (9,10).
- Chandrasekharappa, S.C. et al. (1997) Science 276, 404-7.
- Lemmens, I. et al. (1997) Hum Mol Genet 6, 1177-83.
- Hughes, C.M. et al. (2004) Mol Cell 13, 587-97.
- Yokoyama, A. et al. (2004) Mol Cell Biol 24, 5639-49.
- Karnik, S.K. et al. (2005) Proc Natl Acad Sci USA 102, 14659-64.
- Schnepp, R.W. et al. (2006) Cancer Res 66, 5707-15.
- Yokoyama, A. et al. (2005) Cell 123, 207-18.
- Chen, Y.X. et al. (2006) Proc Natl Acad Sci USA 103, 1018-23.
- Agarwal, S.K. et al. (2005) Horm Metab Res 37, 369-74.
- Wu, X. and Hua, X. (2008) Curr Mol Med 8, 805-15.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 5019 ASH2L (D93F6) XP® Rabbit mAb
- 9751 Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb
- 9725 Di-Methyl-Histone H3 (Lys4) (C64G9) Rabbit mAb
- 4499 Histone H3 (D1H2) XP® Rabbit mAb
- 2184 LSD1 (C69G12) Rabbit mAb
- 6956 NF-κB p65 (L8F6) Mouse mAb
- 5741 Vimentin (D21H3) XP® Rabbit mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9998 BSA
- 9999 Nonfat Dry Milk
For Research Use Only. Not For Use In Diagnostic Procedures.