Cell Signaling Technology

Product Pathways - Neuroscience

Plexin A2 (D44D4) Rabbit mAb #6896

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Endogenous 212 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Plexin A2 (D44D4) Rabbit mAb recognizes endogenous levels of total Plexin A2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala460 of human Plexin A2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from neonatal and adult mouse, and fetal rat brain tissue using Plexin A2 (D44D4) Rabbit mAb.

Background

Class 3 secreted semaphorin (Sema3A) is a chemorepellent that acts upon a wide variety of axons. As such, it induces a dramatic redistribution and depolymerization of actin filaments that results in growth cone collapse. Plexins are single membrane-spanning signaling proteins encompassing Plexin A1, A2, A3, and A4. Plexins form a complex with neuropilin-1 and -2 and the cell adhesion protein L1 to form a functional semaphorin receptor (1,2). The GTPase Rnd1 binds to the cytoplasmic domain of Plexin A1 to trigger cytoskeletal collapse. In contrast, the GTPase RhoD blocks Rnd1-mediated Plexin A1 activation and repulsion of sympathetic axons by Sema3A (3).

  1. Pasterkamp, R.J. and Kolodkin, A.L. (2003) Curr Opin Neurobiol 13, 79-89.
  2. Takahashi, T. and Strittmatter, S.M. (2001) Neuron 29, 429-39.
  3. Zanata, S.M. et al. (2002) J Neurosci 22, 471-7.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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