Product Pathways - Apoptosis
FAIM Antibody #6907
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
FAIM Antibody recognizes endogenous levels of total FAIM protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly145 of human FAIM protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with human FAIM (+), using FAIM Antibody.
FAIM (Fas apoptosis inhibitory molecule) was identified as a protein that was inducibly expressed in B lymphocytes resistant to Fas-mediated apoptosis (1). Expression of FAIM inhibits receptor-mediated apoptosis in B cells as well as other cell types (1-3). FAIM is expressed in germinal center B cells, is positively regulated by IRF-4, and is also capable of inducing IRF-4 expression in a feed-forward mechanism (4). FAIM also regulates T cell receptor-mediated apoptosis by modulating Akt activation and Nur77 expression (2). Knockout mice for FAIM show an increased sensitivity to Fas-mediated apoptosis within B and T cells as well as hepatocytes (5). An alternatively spliced form of FAIM, termed FAIM-L, is found predominantly in the brain (6). In the nervous system, the originally identified FAIM does not appear to play a role in apoptosis, but rather can promote neurite outgrowth through the activation of Erk and NF-κB pathways (7). In contrast, FAIM-L does inhibit neuronal cell death triggered by death receptors (3).
- Schneider, T.J. et al. (1999) J Exp Med 189, 949-56.
- Huo, J. et al. (2010) J Biol Chem 285, 11827-35.
- Segura, M.F. et al. (2007) J Neurosci 27, 11228-41.
- Kaku, H. and Rothstein, T.L. (2009) J Immunol 183, 5575-81.
- Huo, J. et al. (2009) Cell Death Differ 16, 1062-70.
- Zhong, X. et al. (2001) Mol Immunol 38, 65-72.
- Sole, C. et al. (2004) J Cell Biol 167, 479-92.
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For Research Use Only. Not For Use In Diagnostic Procedures.