Cell Signaling Technology

Product Pathways - Apoptosis

FAIM Antibody #6907

Applications Reactivity Sensitivity MW (kDa) Source
W H Endogenous 19 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

FAIM Antibody recognizes endogenous levels of total FAIM protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly145 of human FAIM protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with human FAIM (+), using FAIM Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using FAIM Antibody.

Background

FAIM (Fas apoptosis inhibitory molecule) was identified as a protein that was inducibly expressed in B lymphocytes resistant to Fas-mediated apoptosis (1). Expression of FAIM inhibits receptor-mediated apoptosis in B cells as well as other cell types (1-3). FAIM is expressed in germinal center B cells, is positively regulated by IRF-4, and is also capable of inducing IRF-4 expression in a feed-forward mechanism (4). FAIM also regulates T cell receptor-mediated apoptosis by modulating Akt activation and Nur77 expression (2). Knockout mice for FAIM show an increased sensitivity to Fas-mediated apoptosis within B and T cells as well as hepatocytes (5). An alternatively spliced form of FAIM, termed FAIM-L, is found predominantly in the brain (6). In the nervous system, the originally identified FAIM does not appear to play a role in apoptosis, but rather can promote neurite outgrowth through the activation of Erk and NF-κB pathways (7). In contrast, FAIM-L does inhibit neuronal cell death triggered by death receptors (3).

  1. Schneider, T.J. et al. (1999) J Exp Med 189, 949-56.
  2. Huo, J. et al. (2010) J Biol Chem 285, 11827-35.
  3. Segura, M.F. et al. (2007) J Neurosci 27, 11228-41.
  4. Kaku, H. and Rothstein, T.L. (2009) J Immunol 183, 5575-81.
  5. Huo, J. et al. (2009) Cell Death Differ 16, 1062-70.
  6. Zhong, X. et al. (2001) Mol Immunol 38, 65-72.
  7. Sole, C. et al. (2004) J Cell Biol 167, 479-92.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products