Cell Signaling Technology

Product Pathways - Translational Control

DGCR8 (D78E4) Rabbit mAb #6914

Applications Reactivity Sensitivity MW (kDa) Isotype
W H (M) (R) Endogenous 120 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

DGCR8 (D78E4) Rabbit mAb recognizes endogenous levels of total DGCR8 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly83 of human DGCR8 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 and HeLa cells using DGCR8 (D78E4) Rabbit mAb.

Background

Drosha was identified as a nuclear RNase III that catalyzes the initial step of microRNA (miRNA) processing (1). This enzyme processes the long primary transcript pri-miRNAs into stem-looped pre-miRNAs. Interference of Drosha results in the increase of pri-miRNAs and the decrease of pre-miRNAs (1). Drosha exists in a multiprotein complex called Microprocessor along with other components such as DGCR8 (2). Drosha, along with DGCR8, is necessary for miRNA biogenesis (3).

  1. Lee, Y. et al. (2003) Nature 425, 415-9.
  2. Denli, A.M. et al. (2004) Nature 432, 231-5.
  3. Gregory, R.I. et al. (2004) Nature 432, 235-40.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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