Cell Signaling Technology

Product Pathways - Metabolism

GFAT2 (D40C7) Rabbit mAb #6917

Applications Reactivity Sensitivity MW (kDa) Isotype
W H Endogenous 78 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

GFAT2 (D40C7) Rabbit mAb recognizes endogenous levels of total GFAT2 protein. This antibody also cross-reacts with GFAT1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu205 of human GFAT2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from U251 cells using GFAT2 (D40C7) Rabbit mAb.

Background

GFAT1, glutamine:fructose-6-phosphate aminotransferase 1, is the rate-limiting enzyme of the hexosamine biosynthesis pathway (1). This enzyme catalyzes the conversion of fructose-6-phosphate and glutamine to glucosamine-6-phosphate and glutamate (2). The hexosamine biosynthesis pathway generates the building blocks for protein and lipid glycosylation (2). Furthermore, studies suggest that increased activity of this pathway is a contributing factor to hyperglycemia-induced insulin resistance (1,2). GFAT1 is more active in non-insulin-dependent diabetes mellitus (NIDDM) patients (3). Transgenice mice overexpressing this enzyme in skeletal muscle and adipose tissue show an insulin resistance phenotype (4,5). GFAT2, an isoenzyme of GFAT1, was later identified (6, 7). Studies show that the regulation of GFAT2 is different from that of GFAT1, suggesting differential regulation of the hexosamine pathway in different tissues (7).

  1. Niimi, M. et al. (2001) J Hum Genet 46, 566-71.
  2. DeHaven, J.E. et al. (2001) Diabetes 50, 2419-24.
  3. Yki-Järvinen, H. et al. (1999) Life Sci 65, 215-23.
  4. Cooksey, R.C. et al. (1999) Endocrinology 140, 1151-7.
  5. Hebert, L.F. et al. (1996) J Clin Invest 98, 930-6.
  6. Oki, T. et al. (1999) Genomics 57, 227-34.
  7. Hu, Y. et al. (2004) J Biol Chem 279, 29988-93.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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