Cell Signaling Technology

Product Pathways - Phosphatases

CTDSPL2 (D79G11) Rabbit mAb #6932

Applications Reactivity Sensitivity MW (kDa) Isotype
W H R Mk Endogenous 62 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CTDSPL2 (D79G11) Rabbit mAb recognizes endogenous levels of total CTDSPL2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human CTDSPL2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and Hep G2 cells using CTDSPL2 (D79G11) Rabbit mAb.

Background

CTD small phosphatase-like protein 2 (CTDSPL2, HSPC129) is a putative RNA-polymerase II carboxy-terminal domain (CTD) phosphatase (1) that belongs to a small subfamily of CTD phosphatases (2). The CTD of RNA polymerase II contains multiple Y-S-P-T-S-P-S repeats that are phosphorylated during the transcription cycle (3,4). In general, CTD phosphatases regulate the reversible CTD phosphorylation state of RNA-polymerase II at several stages of RNA synthesis and during post-transcriptional modification (4-6). CTDSPL2 has several structural and functional similarities to other CTD phosphatases, including FCP1, SCP1, DULLARD, and UBLCP1 (1,2).

  1. Qian, H. et al. (2007) Mol Cell Biochem 303, 183-8.
  2. Kim, Y. et al. (2007) Proc Natl Acad Sci USA 104, 6596-601.
  3. Corden, J.L. et al. (1985) Proc Natl Acad Sci USA 82, 7934-8.
  4. Ahn, S.H. et al. (2004) Mol Cell 13, 67-76.
  5. Dahmus, M.E. (1996) J Biol Chem 271, 19009-12.
  6. Goodrich, J.A. and Tjian, R. (1994) Cell 77, 145-56.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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