Product Pathways - Translational Control
GβL (86B8) Rabbit mAb (Magnetic Bead Conjugate) #6938
PhosphoSitePlus® protein, site, and accession data: LST8
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| IP | H M R Mk | Endogenous | 37 | Rabbit IgG |
Applications Key:
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 6938:
- Immunoprecipitation
Specificity / Sensitivity
GβL (86B8) Rabbit mAb (Magnetic Bead Conjugate) detects endogenous levels of total GβL protein.
Source / Purification
GβL (86B8) Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln210 of human GβL.
IP
Immunoprecipitation of extracts from 293 cells using GβL (86B8) Rabbit mAb (Magnetic Bead Conjugate) (lane 1). Rabbit (DA1E) mAb IgG XP® Isotype Control (Magnetic Bead Conjugate) #8726 was used as a negative control (lane 2). The western blot was probed using GβL (86B8) Rabbit mAb #3274 and detected with Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb #3678 followed by Anti-mouse IgG, HRP-linked Antibody #7076.
Description
This Cell Signaling Technology (CST) antibody is immobilized by the covalent reaction of hydrazinonicotinamide-modifed antibody with formylbenzamide-modified magnetic bead. GβL (86B8) Rabbit mAb (Magnetic Bead Conjugate) is useful for immunoprecipitation assays of GβL protein. The unconjugated GβL (86B8) Rabbit mAb #3274 reacts with human, mouse, rat, and monkey GβL protein. CST expects that GβL (86B8) Rabbit mAb (Magnetic Bead Conjugate) will also recognize GβL in these species.
Background
Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian Target of Rapamycin (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL, and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, and rictor (1). GβL associates with the kinase domain of mTOR and stimulates mTOR kinase activity (2). A reduction in GβL expression has been shown to decrease in vivo phosphorylation of S6K1 (2).
- Sarbassov, D.D. et al. (2004) Curr Biol. 14, 1296-1302.
- Kim, D.H. et al. (2003) Mol. Cell 11, 895-904.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
