Product Pathways - Neuroscience
PINK1 (D8G3) Rabbit mAb #6946
PhosphoSitePlus® protein, site, and accession data: PINK1
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H | Endogenous | 60, 50 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
PINK1 (D8G3) Rabbit mAb recognizes endogenous levels of total PINK1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro140 of human PINK1 protein.
Western Blotting
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a cDNA construct expressing full-length human PINK1 (hPINK1, +) using PINK1 (D8G3) Rabbit mAb.
Western Blotting
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with CCCP (10 μM, 24 hr; +), using PINK1 (D8G3) Rabbit mAb.
Background
PTEN induced putative kinase 1, PINK1, is a mitochondrial serine/threonine kinase involved in the normal function and integrity of mitochondria, as well as in reduction of cytochrome c release from mitochondria (1-3). PINK1 phosphorylates Parkin and promotes its translocation to mitochondria (2). Research studies have shown that mutations in PINK1 are linked to autosomal recessive early onset Parkinson’s disease, and are associated with loss of protective function, mitochondrial dysfunction, aggregation of α-synuclein, as well as proteasome dysfunction (1,3).
- Liu, W. et al. (2009) PLoS One 4, e4597.
- Kim, Y. et al. (2008) Biochem Biophys Res Commun 377, 975-80.
- Petit, A. et al. (2005) J Biol Chem 280, 34025-32.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
