Cell Signaling Technology

Product Pathways - Translational Control

CNOT2 Antibody #6955

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 62 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CNOT2 Antibody recognizes endogenous levels of total CNOT2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CNOT2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CNOT2 Antibody.

Background

The evolutionarily conserved CCR4-NOT (CNOT) complex regulates mRNA metabolism in eukaryotic cells (1). This regulation occurs at different levels of mRNA synthesis and degradation, including transcription initiation, elongation, deadenylation, and degradation (1). Multiple components, including CNOT1, CNOT2, CNOT3, CNOT4, CNOT6, CNOT7, CNOT8, CNOT9, and CNOT10 have been identified in this complex (2). In addition, subunit composition of this complex has been shown to vary among different tissues (3).

  1. Denis, C.L. and Chen, J. (2003) Prog Nucleic Acid Res Mol Biol 73, 221-50.
  2. Lau, N.C. et al. (2009) Biochem J 422, 443-53.
  3. Chen, C. et al. (2011) Biochem Biophys Res Commun 411, 360-4.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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