Cell Signaling Technology

Product Pathways - Development

TACE (D22H4) Rabbit mAb #6978

Applications Reactivity Sensitivity MW (kDa) Isotype
W H Endogenous 135 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TACE (D22H4) Rabbit mAb recognizes endogenous levels of total TACE protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human TACE protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from Raji and Jurkat cells, untreated or treated with peptide N-glycosidase F (PNGase F), using TACE (D22H4) Rabbit mAb.

Background

TACE (TNF-α converting enzyme), also known as ADAM17, is a transmembrane metalloprotease that plays a key role in the cleavage of a number cell surface molecules in a process known as “shedding". TACE is abundantly expressed in many adult tissues, but in fetal development expression is differentially regulated (1). An important substrate of TACE is pro-TNF-α (1). Increased expression of TACE is associated with several pathological conditions including osteoarthritis and rheumatoid arthritis, where the pro-inflammatory effects of increased TNF-α contribute to disease pathogenesis (2,3). Regulation of other important molecules by TACE such as EGFR and Notch has recently been documented. TACE is responsible for the shedding of EGFR ligands such as amphiregulin and TNF-α. Some tumors have hyperactivated EGFR due to upregulated TNF-α production and upregulated TACE, making TACE a potential target for drug development (4). TACE activates Notch in a ligand-independent manner and has been shown to play a role in the development of the Drosophila nervous system (5). TACE has also been proposed to act as α-secretase for amyloid precursor protein (APP) (6), and to be involved in the renewal and proliferation of neural stem cells (7).

  1. Black, R.A. et al. (1997) Nature 385, 729-33.
  2. Amin, A.R. (1999) Osteoarthritis Cartilage 7, 392-4.
  3. Patel, I.R. et al. (1998) J Immunol 160, 4570-9.
  4. Kenny, P.A. (2007) Differentiation 75, 800-8.
  5. Delwig, A. and Rand, M.D. (2008) Cell Mol Life Sci 65, 2232-43.
  6. Deuss, M. et al. (2008) Curr Alzheimer Res 5, 187-201.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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