Cell Signaling Technology

Product Pathways - TGF-beta/Smad Signaling

BMPR2 Antibody #6979

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M Endogenous 150 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

BMPR2 Antibody recognizes endogenous levels of total BMPR2 protein. This antibody may also detect a 115 Kd unspecific band.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys763 of human BMPR2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using BMPR2 Antibody.

Background

BMPR2 is a type II serine/threonine receptor kinase that binds to an array of secreted bone morphogenetic proteins (BMPs). BMPs belong to the superfamily of TGF-β ligands that modulate gastrulation, neurogenesis, chondrogenesis, interdigital cell death, and bone morphogenesis (1-5). In contrast to the TGF-β type II receptor, BMPR2 contains an extended carboxyl-terminal region that interacts with multiple signaling molecules to modulate the responsiveness of target genes to BMPs (6,7). BMP signaling requires oligomerization of both type I and type II receptors to elicit a functional response of target genes. BMP binding to type I and II receptors induces Smad1/5/8 phosphorylation which is required for the activation of target genes (7). In vitro and in vivo evidence suggests that defects in BMPR2 may contribute to pulmonary hypertension, inflammation, and endothelial injury (8,9).

  1. Storm, E.E. et al. (1994) Nature 368, 639-43.
  2. Storm, E.E. and Kingsley, D.M. (1996) Development 122, 3969-79.
  3. Reddi, H. (1995) Adv Dent Res 9, 13.
  4. Zhang, H. and Bradley, A. (1996) Development 122, 2977-86.
  5. Dunn, N.R. et al. (1997) Dev Biol 188, 235-47.
  6. Kudo, T.A. et al. (2010) Tohoku J Exp Med 222, 121-9.
  7. Gilboa, L. et al. (2000) Mol Biol Cell 11, 1023-35.
  8. Machado, R.D. et al. (2001) Am J Hum Genet 68, 92-102.
  9. Long, L. et al. (2009) Circulation 119, 566-76.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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