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Render Timestamp:
6/9/2026, 6:18:04 AM EDT
6/9/2026, 10:18:04 AM UTC
Commit: 33ae406c5df2bbc7a7d3c17605228fbeff8ea416
XML generation date: 2025-03-07 13:11:15.273
Product last modified at: 2025-12-21T22:57:10.533Z
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PDP - Template Name: Growth Factors and Cytokines
PDP - Template ID: *******9ad1159
  1. Products /
  2. Cytokines & Growth Factors /
  3. Mouse His6Interleukin-33 (mHis6IL-33)

Mouse His6Interleukin-33 (mHis6IL-33) #6983

Inquiry Info. # 6983

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    Product Information

    Formulation

    With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 1 mM EDTA, 3 mM DTT and 20 μg BSA per 1 μg mHis6IL-33. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 1 mM EDTA and 3 mM DTT.

    Storage

    Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles. Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

    Product Description

    MW (kDa) 22
    Purity >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mHis6IL-33. All lots are greater than 98% pure.
    Endotoxin Less than 0.01 ng endotoxin/1 μg mHis6IL-33.
    Activity The bioactivity of recombinant mHis6IL-33 was determined by its ability to induce IL-13 production by MC/9 cells. The ED50 of each lot is between 1-6 ng/ml.
    Molecular Formula Recombinant N-terminally His6-tagged mIL-33 has a calculated MW of 19,439. DTT-reduced and non-reduced protein migrate as 22 kDa polypeptides.

    Source / Purification

    Recombinant mouse His6IL-33 (mHis6IL-33) Ser109-Ile266 (Accession #NP_598536) was produced in E.coli at Cell Signaling Technology.

    Background

    IL-33 is a novel member of the IL-1 family of proteins that was discovered in a computational search to identify unknown family members (1). IL-33 is expressed by fibroblasts and endothelial cells, and is upregulated in response to inflammatory stimuli (1). IL-33 plays a key role in Th2 type inflammatory responses, including atopic allergic responses and asthma (1,2). The IL-33 receptor consists of the ST2 and IL-1 accessory protein (IL-1-Acp) (1,3). ST2 is expressed on mast cells, basophils and Th2 cells (1). In addition to its putative role as a Th2 cytokine, IL-33 also contains a nuclear localization domain and may function as a nuclear factor (4,5). The nuclear function of IL-33 remains unclear. IL-33 is released by cellular necrosis, similar to other “alarmins”, thereby signaling tissue injury (5,6). However, more recent data suggests that IL-33 may also be actively secreted from live cells via a non-classical mechanism (7).

    Database Links

    UniProt ID: Q8BVZ5


    Entrez-Gene Id: 77125


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