Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Mouse His6Interleukin-33 (mHis6IL-33) #6983

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Source

Recombinant mouse His6IL-33 (mHis6IL-33) Ser109-Ile266 (Accession #NP_598536) was produced in E.coli at Cell Signaling Technology.

Molecular Characterization

Recombinant N-terminally His6-tagged mIL-33 has a calculated MW of 19,439. DTT-reduced and non-reduced protein migrate as 22 kDa polypeptides.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mHis6IL-33. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant mHis6IL-33 was determined by its ability to induce IL-13 production by MC/9 cells. The ED50 of each lot is between 1-6 ng/ml.

Coomassie Gel

Coomassie Gel

The purity of recombinant mHis6IL-33 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mHis6IL-33 and staining overnight with Coomassie Blue.

Bioactivity

Bioactivity

The production of IL-13 by MC/9 cells cultured with increasing concentrations of mHis6IL-33 was assessed. Media from cells incubated with mHis6IL-33 for 24 hours was collected and assayed for IL-13 production by ELISA and the OD450 was determined.

Endotoxin

Less than 0.01 ng endotoxin/1 μg mHis6IL-33.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 1 mM EDTA, 3 mM DTT and 20 μg BSA per 1 μg mHis6IL-33. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 1 mM EDTA and 3 mM DTT.

Background

IL-33 is a novel member of the IL-1 family of proteins that was discovered in a computational search to identify unknown family members (1). IL-33 is expressed by fibroblasts and endothelial cells, and is upregulated in response to inflammatory stimuli (1). IL-33 plays a key role in Th2 type inflammatory responses, including atopic allergic responses and asthma (1,2). The IL-33 receptor consists of the ST2 and IL-1 accessory protein (IL-1-Acp) (1,3). ST2 is expressed on mast cells, basophils and Th2 cells (1). In addition to its putative role as a Th2 cytokine, IL-33 also contains a nuclear localization domain and may function as a nuclear factor (4,5). The nuclear function of IL-33 remains unclear. IL-33 is released by cellular necrosis, similar to other “alarmins”, thereby signaling tissue injury (5,6). However, more recent data suggests that IL-33 may also be actively secreted from live cells via a non-classical mechanism (7).

  1. Liew, F.Y. et al. (2010) Nat Rev Immunol 10, 103-10.
  2. Chackerian, A.A. et al. (2007) J Immunol 179, 2551-5.
  3. Borish, L. and Steinke, J.W. (2011) Curr Allergy Asthma Rep 11, 7-11.
  4. Moussion, C. et al. (2008) PLoS One 3, e3331.
  5. Cayrol, C. and Girard, J.P. (2009) Proc Natl Acad Sci U S A 106, 9021-6.
  6. Lüthi, A.U. et al. (2009) Immunity 31, 84-98.
  7. Kakkar, R. et al. (2012) J Biol Chem 287, 6941-8.

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