Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-APP (Thr668) (D90B8) Rabbit mAb #6986

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Endogenous 100-140 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-APP (Thr668) (D90B8) Rabbit mAb detects different isoforms of endogenous amyloid β (A4) precursor protein only when phosphorylated at Thr668 (or the corresponding position of other isoforms).

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr668 of human APP protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or nocodazole-treated (1 μg/ml, 18 hr) using Phospho-APP (Thr668) (D90B8) Rabbit mAb (upper) or APP Antibody #2452 (lower).

Background

Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms (1). The amino acid sequence of APP contains the amyloid domain, which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released Aβ fragments form the main components of amyloid plaques in Alzheimer's disease (AD) (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion of this protein (2-5). Phosphorylation at Thr668 (a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell-cycle dependent and peaks during G2/M phase (4). APP phosphorylated at Thr668 exists in adult rat brain and correlates with cultured neuronal differentiation (5,6).

  1. Selkoe, D.J. (1996) J Biol Chem 271, 18295-8.
  2. Caporaso, G.L. et al. (1992) Proc Natl Acad Sci USA 89, 3055-9.
  3. Hung, A.Y. and Selkoe, D.J. (1994) EMBO J 13, 534-42.
  4. Suzuki, T. et al. (1994) EMBO J 13, 1114-22.
  5. Ando, K. et al. (1999) J Neurosci 19, 4421-7.
  6. Iijima, K. et al. (2000) J Neurochem 75, 1085-91.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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