Cell Signaling Technology

Product Pathways - Development

MAML2 (D41E6) Rabbit mAb #6988

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Endogenous 160 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MAML2 (D41E6) Rabbit mAb detects endogenous levels of total MAML2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp800 of human MAML2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 and SH-SY5Y cells using MAML2 (D41E6) Rabbit mAb.

Background

Mastermind-like (MAML) family of proteins are homologs of Drosophila Mastermind. The family is composed of three members in mammals: MAML1, MAML2, and MAML3 (1,2). MAML proteins form complexes with the intracellular domain of Notch (ICN) and the transcription factor CSL (RBP-Jκ) to regulate Notch target gene expression (3-5). MAML1 also interacts with myocyte enhancer factor 2C (MEF2C) to regulate myogenesis (6). MAML2 is frequently found to be fused with Mucoepidermoid carcinoma translocated gene 1 (MECT1, also know as WAMTP1 or TORC1) in patients with mucoepidermoid carcinomas and Warthin's tumors (7).

  1. Wu, L. et al. (2002) Mol. Cell Biol. 22, 7688-7700.
  2. Lin, S.E. et al. (2002) J. Biol. Chem. 277, 50612-50620.
  3. Kitagawa, M. et al. (2001) Mol. Cell Biol. 21, 4337-4346.
  4. Nam, Y. et al. (2003) J. Biol. Chem. 278, 21232-21239.
  5. Nam, Y. et al. (2006) Cell 124, 973-983.
  6. Shen, H. et al. (2006) Genes Dev. 20, 675-688.
  7. Tonon, G. et al. (2003) Nat. Genet. 33, 208-213.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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