Secondary Antibodies
Anti-rabbit IgG F(ab')2 Fragment (Sepharose Bead Conjugate) #6990
Protocols
- 6990:
- Immunoprecipitation
Specificity / Sensitivity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, Rabbit IgG and Rabbit serum. No reaction was observed against anti-pepsin, anti-goat IgG Fc, or human or mouse serum proteins.
Source / Purification
Anti-rabbit IgG F(ab')2 Fragment is produced by immunizing goats with rabbit IgG whole molecules. The Anti-rabbit IgG F(ab')2 Fragment is prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption to remove any unwanted reactivities, pepsin digestion, and chromatographic separation.
IP
Immunoprecipitation of Jurkat cell extracts using Akt (pan) (C67E7) Rabbit mAb #4691. Extracts were incubated with either Anti-Rabbit IgG F(ab')2 Fragment (Sepharose Bead Conjugate) (lane 1) or Protein A Beads (lane 2). The western blot was probed using Akt (pan) (40D4) Mouse mAb #2920.
Description
This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Anti-rabbit IgG F(ab') 2 Fragment (Sepharose Bead Conjugate) is useful for the immunoprecipitation of antibodies raised in rabbit.
Directions for Use
Add 10-20 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg/ml in 1X Cell Lysis Buffer (10X) #9803. See protocol for more details.
Background
Anti-rabbit IgG F(ab')2 Fragment (Sepharose Bead Conjugate) is ideal for immunoprecipitation. This secondary antibody will detect rabbit antibodies. Since this secondary antibody is made with F(ab')2 fragments, non-specific binding through Fc receptors present on cells will be eliminated.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
