Cell Signaling Technology

Product Pathways - Neuroscience

Nestin (Rat-401) Mouse mAb (Alexa Fluor® 488 Conjugate) #6994

Applications Reactivity Sensitivity Isotype
IF-F R Endogenous Mouse IgG1

Applications Key:  IF-F=Immunofluorescence (Frozen)
Reactivity Key:  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Nestin (Rat-401) Mouse mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of rat nestin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with E15 rat spinal cord.

IF-F

IF-F

Confocal immunofluorescent analysis of P1 rat cerebellum (left) and adult rat cerebellum (right) using Nestin (Rat-401) Mouse mAb (Alexa Fluor® 488 Conjugate) (green) and Neurofilament-L (C28E10) Rabbit mAb #2837 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct immunofluorescent analysis in rat tissue. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Nestin (Rat-401) Mouse mAb #4760.

Background

Nestin is an intermediate filament family member protein that is structurally related to the neurofilament proteins (1). It is highly expressed in the developing brain, where it may help to regulate cell structure and intracellular processes required for neural cell division and migration (1,2). Upon maturation of the brain, nestin expression is quickly down-regulated and replaced by expression of the neurofilament proteins (2). Because nestin is expressed in both mature and precursor neuronal and glial cells, as well as in the developing brain and in the brain and spinal cord following damage, nestin is widely accepted as a marker of neural stem/progenitor cells (3). Research studies have shown that expression of nestin is also found in cells from various nervous system tumors, including gliomas, neuroblastomas, astrocytomas, and it is generally accepted as a marker for neural cancer stem cells (3). However, nestin expression has also been observed in astrocytes, retina, cardiac muscle, pancreas, and other tissues (3). Therefore, the acceptance of nestin as an exclusive marker of neural stem/progenitor cells is not unanimous.

  1. Michalczyk, K. and Ziman, M. (2005) Histol Histopathol 20, 665-71.
  2. Giliarov, A.V. (2007) Morfologiia 131, 85-90.
  3. Gilyarov, A.V. (2008) Neurosci Behav Physiol 38, 165-9.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products