Cell Signaling Technology

Product Pathways - Neuroscience

Merlin (D1D8) Rabbit mAb #6995

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Endogenous 70 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Merlin (D1D8) Rabbit mAb recognizes endogenous levels of total Merlin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Merlin protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from fetal rat brain, and both wild type and NF2(-/-) mouse embryonic fibroblasts (MEFs) using Merlin (D1D8) Rabbit mAb (upper) and Ezrin/Radixin/Moesin Antibody #3142 (lower). (MEF wt and MEF NF2(-/-) cells were kindly provided by Dr. Andrea McClatchey and Dr. Andrew Gladden, MGH Cancer Center and Harvard Medical School, Charlestown MA).

Background

Neurofibromatosis 2 (NF2) is an autosomal dominant inherited disorder that is characterized by the occurrence of vestibular schwannomas, meningiomas, and other nervous system tumors. Both the familial tumors of NF2 and equivalent sporadic tumors in the general population are caused by inactivation of the NF2 tumor suppressor gene. Merlin (moesin, ezrin, and radixin like protein), the NF2 gene product, has striking similarity to ezrin, radixin, and moesin (ERM). Regulation of merlin (aka schwannomin) and ERM proteins involves intramolecular and intermolecular head-to-tail associations between family members (1). Merlin and ERM proteins act as linkers between the plasma membrane and the cytoskeleton, affecting cell morphology, polarity, and signal transduction (2). Merlin is phosphorylated by the Rac/Cdc42 effector p21-activated kinase (PAK) at Ser518, negatively regulating Rac (3,4).

  1. Ramesh, V. (2004) Nat. Rev. Neurosci. 5, 462-470.
  2. Bretscher, A. et al. (2002) Nat. Rev. Mol. Cell Biol. 3, 586-599.
  3. Xiao, G. H. et al. (2002) J. Biol. Chem. 277, 883-886.
  4. Kissil, J. L. et al. (2003) Mol. Cell 12, 841-849.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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