Product Pathways - NF-kB Signaling
RAGE 1 (D1A12) Rabbit mAb #6996
PhosphoSitePlus® protein, site, and accession data: RAGE
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H (Mk) | Endogenous | 55 | Rabbit IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 6996:
- Western Blotting
Specificity / Sensitivity
RAGE 1 (D1A12) Rabbit mAb recognizes endogenous levels of total RAGE 1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human RAGE 1 protein.
Western Blotting
Western blot analysis of extracts from human lung tissue using RAGE 1 (D1A12) Rabbit mAb.
Background
The receptor for advanced glycation end products (RAGE) is member of the immunoglobulin (Ig) superfamily. It can be expressed as full-length, membrane-bound RAGE isoform 1 or as a secreted sRAGE protein that lacks a transmembrane domain (1). RAGE is detected during early developmental stages and in the lung under normal physiological conditions (2) and is upregulated at sites of inflammation (3). Advanced glycation end products (AGEs) and a variety of other ligands interact with this receptor (1). Ligand binding activates full-length RAGE and initiates downstream signaling pathways that include activation of NF-κB, which leads to production of pro-inflammatory cytokines and inflammation (4). Activation of these pathways has been implicated in various disease states including Alzheimer disease, diabetes, arthritis, and atherosclerosis (4). Soluble RAGE can competitively bind RAGE ligands in the extracellular environment, which prevents ligand interaction with full-length RAGE at the cell surface (1).
- Bierhaus, A. et al. (2005) J Mol Med 83, 876-86.
- Brett, J. et al. (1993) Am J Pathol 143, 1699-712.
- Sparvero, L.J. et al. (2009) J Transl Med 7, 17.
- Lin, L. et al. (2009) Front Biosci 14, 1403-13.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 4800 RAGE 1 Antibody
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- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
