Product Pathways - PathScan ELISA
PathScan® Sandwich ELISA Lysis Buffer (1X) #7018
|7018S||30 ml||---||In Stock||---|
|7018||carrier free and custom formulation / quantity||email request|
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PathScan® Sandwich ELISA Lysis buffer is used to create cell extract for ELISA applications.
Directions for Use
1. If buffer will be used regularly, it is recommended that the 1X buffer be kept at 4ºC for 1-2 weeks. For longer periods of time, buffer should be stored at -20ºC. Aliquotting of 1X buffer is recommended to avoid freeze thaw cycles.
2. Thaw 1X buffer at 24-30ºC, mixing end-over-end.
3. Chill 1X buffer on ice and add PMSF just prior to use.
Solutions and Reagents
1X Lysis Buffer: 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% Triton, 20 mM sodium Pyrophosphate, 25 mM Sodium Fluoride, 1 mM β-glycerophosphate, 1 mM Na3VO4, 1 µg/ml leupeptin
Note: Cell Signaling Technology recommends adding 1 mM PMSF immediately before use.
PathScan® Sandwich ELISA Lysis buffer (1X) was developed for ELISA applications and is formulated to better inhibit Serine / Threonine class of phosphatases after cell extracts are made.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.
PathScan® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.