Buffers
PathScan® Sandwich ELISA Lysis Buffer (1X) #7018
Description
PathScan® Sandwich ELISA Lysis buffer is used to create cell extract for ELISA applications.1X Cell Lysis Buffer ingredients:1 mM β-glycerophosphate (CAS# 819-83-0)1 mM EDTA disodium salt (CAS# 6381-92-6)1 mM EGTA (CAS# 64-42-5)1 µg/ml leupeptin (CAS# 103476-89-7)150 mM sodium chloride (CAS# 7647-14-5)20 mM sodium Pyrophosphate (CAS# 13472-36-1)25 mM Sodium Fluoride (CAS# 007681-49-4)1 mM sodium Orthovanadate (CAS# 13721-39-6)20 mM Tris-Cl (CAS# 1185-53-1)Triton X-100 (CAS# 9036-19-5)
Directions for Use
1. If buffer will be used regularly, it is recommended that the 1X buffer be kept at 4ºC for 1-2 weeks. For longer periods of time, buffer should be stored at -20ºC. Aliquotting of 1X buffer is recommended to avoid freeze thaw cycles.
2. Thaw 1X buffer at 24-30ºC, mixing end-over-end.
3. Chill 1X buffer on ice and add PMSF just prior to use.
Solutions and Reagents
20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM disodium EDTA, 1 mM EGTA, 1% Triton, 20 mM sodium pyrophosphate, 25 mM sodium fluoride, 1 mM β-glycerophosphate, 1 mM Na3VO4, 1 µg/ml leupeptin
Note: Cell Signaling Technology recommends adding 1 mM PMSF immediately before use.
Background
PathScan® Sandwich ELISA Lysis buffer (1X) was developed for ELISA applications and is formulated to better inhibit Serine / Threonine class of phosphatases after cell extracts are made.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
