Cell Signaling Technology

Product Pathways - Neuroscience

NeuroD (D90G12) Rabbit mAb #7019

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 49 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

NeuroD (D90G12) Rabbit mAb detects endogenous levels of total NeuroD protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human NeuroD protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from human cerebellum using NeuroD (D90G12) Rabbit mAb.

Background

NeuroD is a member of the basic helix-loop-helix (bHLH) family of transcription factors. These proteins function by forming heterodimers with E-proteins and binding to the canonical E-box sequence CANNTG (1,2). Neuronal activity results in CaMKII-mediated phosphorylation of NeuroD at Ser336, which is necessary for formation and growth of dendrites (3,4). NeuroD is also phosphorylated at Ser274 though the results are context dependent as phosphorylation by Erk stimulates NeuroD activity in pancreatic β-cells while phosphorylation by GSK-3β inhibits NeuroD in neurons (3). NeuroD is crucially important in both the pancreas and developing nervous system, and plays a large role in the development of the inner ear and mammalian retina (3). Mice lacking NeuroD become severely diabetic and die shortly after birth due to defects in β-cell differentiation (2,3,5,6). The lack of NeuroD in the brain results in severe defects in development (5). Human mutations have been linked to a number of types of diabetes including type I diabetes mellitus and maturity-onset diabetes of the young (1,3).

  1. Schonhoff, S.E. et al. (2004) Endocrinology 145, 2639-2644.
  2. Sharma, A. et al. (1999) Mol. Cell Biol. 19, 704-713.
  3. Chae, J.H. et al. (2004) Mol. Cells 18, 271-288.
  4. Gaudillière, B. et al. (2004) Neuron 41, 229-241.
  5. Miyata, T. et al. (1999) Genes Dev. 13, 1647-1652.
  6. Naya, F.J. et al. (1997) Genes Dev. 11, 2323-2334.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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