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Anti-rabbit IgG, HRP-linked Antibody #7074
Description
Affinity purified goat anti-rabbit IgG (H&L) antibody is conjugated to horseradish peroxidase.
Applications
This product has been optimized for use as a secondary antibody in chemiluminescent Western blotting applications.
Background
Chemiluminescent detection systems have emerged as the best all-around method for detection of Western blots. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives. Because results are generated on film, it is possible to record and store data permanently, and blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. Horseradish peroxidase (HRP) conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. Horseradish peroxidase-antibody conjugates have a very high turnover rate, giving good sensitivity with short reaction times.
Application References
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