Secondary Antibodies
Anti-biotin, HRP-linked Antibody #7075
Specificity / Sensitivity
This antibody may cross react with endogenously expressed biotinylated proteins. Careful titration may be needed.
Description
Affinity purified goat anti-biotin antibody is conjugated to horseradish peroxidase.
Applications
This product has been optimized to detect biotinylated protein markers.
Background
Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 7727 Biotinylated Protein Ladder Detection Pack
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
For Research Use Only. Not For Use In Diagnostic Procedures.
