Product Pathways - Translational Control
HIF-2α (D9E3) Rabbit mAb #7096
PhosphoSitePlus® protein, site, and accession data: HIF2A
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H | Endogenous | 120 | Rabbit IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
HIF-2α (D9E3) Rabbit mAb recognizes endogenous levels of total HIF-2α protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala758 of human HIF-2α protein.
Western Blotting
Western blot analysis of Hep G2 cells, untreated (-) or treated with cobalt chloride (CoCl2, 0.1 mM, 4 hr, +) using HIF-2α (D9E3) Rabbit mAb (upper) or β-Actin Antibody #4967 (lower).
Background
Hypoxia-inducible factor (HIF) is essential for the cellular response to hypoxia (1,2). Under normoxia conditions, the α subunit of HIF is ubiquitinated by von Hippel-Lindau (VHL) protein and is degraded in the ubiquitin/proteasome pathway (1,2). Hypoxia inhibits the degradation of the α subunit, which leads to its stabilization (1,2). HIF, in turn, regulates the transcription of a variety of genes that respond to hypoxia conditions (1,2). There are several isoforms of the HIF α subunit (2). Studies have found that HIF-1α and HIF-2α expression is increased in some human cancers (2). HIF-1α has both pro- and anti-proliferative activities, whereas HIF-2α does not possess anti-proliferative activity (2). Therefore, HIF-2α likely plays an important role in tumorigenesis (2,3).
- Kaelin, W.G. (2005) Biochem Biophys Res Commun 338, 627-38.
- Toschi, A. et al. (2008) J Biol Chem 283, 34495-9.
- Gordan, J.D. and Simon, M.C. (2007) Curr Opin Genet Dev 17, 71-7.
Application References
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Companion Products
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- 9998 BSA
- 9999 Nonfat Dry Milk
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
