Cell Signaling Technology

Product Pathways - PathScan ELISA

PathScan® Phospho c-Jun (Ser63) Sandwich ELISA Antibody Pair #7141

Kit Includes Volume Cap Color
Phospho-c-Jun (Ser63) Capture Antibody (100X) 0.4 milliliters Pink
c-Jun Detection Antibody (100X) 0.4 milliliters Blue
Anti-Mouse IgG HRP-Linked Antibody (1000X) 0.04 milliliters Yellow

Capture and Detection Antibodies are stored at 4°C. HRP-Linked Secondary Antibody is stored at -20°C.

Species Cross-Reactivity

H M R

Reactivity Key:  H=Human  M=Mouse  R=Rat

Description

CST's PathScan® Phospho-c-Jun (Ser63) Sandwich ELISA Antibody Pair is being offered as an alternative to our PathScan® Phospho-c-Jun (Ser63) Sandwich ELISA Kit #7145. Capture and Detection antibodies (100X stocks) and a HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are provided for performing 4 x 96 well ELISAs. Phospho-c-Jun (Ser63) Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added, followed by c-Jun Detection Antibody and HRP-conjugated secondary antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance at 450 nm is proportional to the quantity of phospho-c-jun (Ser63) protein.*Antibodies in this kit are custom formulations specific to the kit.

Sandwich ELISA

Sandwich ELISA

The relationship between lysate protein concentration from untreated and UV treated 293 cells and the absorbance at 450 nm using PathScan® Phospho-c-Jun (Ser 63) Sandwich ELISA Antibody Pair #7141 is shown. 293 cells were UV-treated, allowed to recover for 30 minutes at 37°C and then lysed.

Background

c-Jun is a component of the transcription factor AP-1, which binds and activates transcription at TRE/AP-1 elements. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 (1,2). Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun-dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress-induced signaling pathways. The MAP kinase homologue SAPK/JNK binds to the amino-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun (3,4).

  1. Binetruy, B. et al. (1991) Nature 351, 122-127.
  2. Smeal, T. et al. (1991) Nature 354, 494-496.
  3. Derijard, B. et al. (1994) Cell 76, 1025-1037.
  4. Kyriakis, J.M. et al. (1994) Nature 369, 156-160.

Application References

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