Product Pathways - PathScan ELISA
PathScan® Total Survivin Sandwich ELISA Kit #7169
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Important Ordering Details: Product is assembled upon order to ensure maximum activity. Domestic: Please allow up to two weeks for your order to be processed and shipped. International: Please allow up to three weeks, depending on the country, for your order to be processed and shipped.
|Kit Includes||Volume||Solution Color|
|Survivin mAb Coated Microwells||96 tests|
|Survivin Detection Ab||11 ml||Green|
|Anti-rabbit IgG, HRP-linked Antibody||11 ml||Red|
|TMB Substrate #7004||11 ml||Colorless|
|STOP Solution #7002||11 ml||Colorless|
|Sealing Tape||2 sheets|
|ELISA Wash Buffer (20X)||25 ml||Colorless|
|ELISA Sample Diluent||25 ml||Blue|
|Cell Lysis Buffer (10X) #9803||15 ml||Yellowish|
Note: 12 8-well modules –Each module is designed to break apart for 8 tests.
Note: Kit should be stored at 4°C with the exception of Cell Lysis Buffer, which is stored at –20°C (packaged separately).
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
CST's PathScan® Total Survivin Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of total survivin protein. A Survivin Mouse mAb has been coated onto the microwells. After incubation with cell lysates, both phospho and nonphospho survivin proteins are captured by the coated antibody. Following extensive washing, Survivin Rabbit Detection Antibody is added to detect the captured survivin protein. Anti-rabbit IgG, HRP-linked Antibody #7074 is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of absorbance for this developed color is proportional to the quantity of total survivin protein.Antibodies in kit are custom formulations specific to kit.
Specificity / Sensitivity
CST's PathScan® Total Survivin Sandwich ELISA Kit #7169 detects endogenous levels of total survivin protein. As shown in Figure 1, survivin protein from human (HeLa, H526 and MCF-7) and monkey (COS), is detected by this kit. In Figure 3, Western analysis of protein captured in microwells coated with the survivin capture antibody shows a major band corresponding to the survivin protein (data not shown). This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
ELISA - Western correlation
Figure 1: Survivin protein from human cells (HeLa, H526 and MCF-7) and monkey cells (COS) can be detected by PathScan® Total Survivin Sandwich ELISA Kit #7169. However, this kit cannot detect the survivin protein from other species, such as mink (MV1LU), rat (C6), mouse (BAF3) and hamster (CHO) cell lines. The absorbance readings at 450 nm are shown in the top figure, while the corresponding Western blot using Survivin (6E4) Mouse mAb #2802, is shown in the bottom figure.
Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (1). Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle by inhibiting apoptosis and promoting cell division (2,3). This regulatory process requires the phosphorylation of survivin at Thr34 by p34 cdc2 kinase (4). Gene targeting using a Thr34 phosphorylation-defective survivin mutant, as well as antisense survivin, have been shown to inhibit tumor growth (5,6).
- Reed, J.C. and Reed, S.I. (1999) Nature Cell Biol. 1, 199-200.
- Li, F. et al. (1998) Nature 396, 580-584.
- Li, F. et al. (1999) Nat. Cell Biol. 1, 461-466.
- O'Connor, D.S. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 13103-13107.
- Olie, R.A. et al. (2000) Cancer Res. 60, 2805-2809.
- Grossman, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 635-640.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 2808 Survivin (71G4B7) Rabbit mAb
- 2810 Survivin (71G4B7) Rabbit mAb (Alexa Fluor® 488 Conjugate)
- 2803 Survivin Antibody
- 2802 Survivin (6E4) Mouse mAb
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 9803 Cell Lysis Buffer (10X)
- 9808 Phosphate Buffered Saline (PBS-20X)
- 9809 Phosphate Buffered Saline with Tween 20 (PBST-20X)
- 7002 STOP Solution
- 7004 TMB Substrate
For Research Use Only. Not For Use In Diagnostic Procedures.