Product Pathways - PathScan ELISA
PathScan® Acetyl-Histone H2B Sandwich ELISA Kit #7178
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Important Ordering Details: Product is assembled upon order to ensure maximum activity. Domestic: Please allow up to two weeks for your order to be processed and shipped. International: Please allow up to three weeks, depending on the country, for your order to be processed and shipped.
|Kit Includes||Volume||Solution Color|
|Histone H2B Ab CoatedMicrowells||96 tests|
|Acetylated-Lysine Detection Ab||11 ml||Green|
|Anti-rabbit IgG, HRP-linked Antibody||11 ml||Red|
|TMB Substrate #7004||11 ml||Colorless|
|STOP Solution #7002||11 ml||Colorless|
|Sealing Tape||2 sheets|
|ELISA Wash Buffer (20X)||25 ml||Colorless|
|Sample Diluent||25 ml||Blue|
|Cell Lysis Buffer (10X) #9803||15 ml||Yellowish|
Note: 12 8-well modules –Each module is designed to break apart for 8 tests.
Note: Kit should be stored at 4°C with the exception of Cell Lysis Buffer, which is stored at –20°C (packaged separately).
H M Mk
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
The PathScan® Acetyl-Histone H2B Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of acetylated lysines on Histone H2B. A Histone H2B Antibody has been coated onto the microwells. After incubation with cell lysates, Histone H2B is captured by the coated antibody. Following extensive washing, an Acetylated-Lysine Rabbit mAb is added to detect the acetylated lysines on the Histone H2B protein. Anti-rabbit IgG, HRP linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB is added to develop color. The magnitude of optical density for this developed color is proportional to the quantity of acetylated Histone H2B.Antibodies in kit are custom formulations specific to kit.
Specificity / Sensitivity
CST's PathScan® Acetyl-Histone H2B Sandwich ELISA Kit detects endogenous levels of acetylated Histone H2B. Using this Sandwich ELISA Kit #7178, acetylated lysines on Histone H2B are detected when treated with TSA in Cos cells. However, the levels of total Histone H2B protein remain unchanged, as shown by Western blot analysis, using the Histone H2B Antibody #2722 (figure 1). Jurkat cells treated with TSA show similar results (data not shown). This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
ELISA - Western correlation
Figure 1: Treatment of COS cells with TSA causes accumulation of acetylation on Histone H2B, detected by Sandwich ELISA Kit #7178, but does not affect the level of total histone H2B protein, detected by Western analysis. OD 450 readings are shown in the top figure, while the corresponding Western blots using the Acetylated Lysine Mouse mAb (Ac-K-103) #9681 (left panel) or Histone H2B Antibody #2722 (right panel), are shown in the bottom figure.
Figure 2: The relationship between protein concentration of lysates from untreated and TSA treated HeLa cells and kit assay optical density readings. HeLa cells (80% confluence) were treated with TSA (4uM overnight) and then lysed. An acid extraction was performed for cell lysis in the presence of 5mM sodium butyrate.
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
- Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
- Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
- Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
- Cheung, P. et al. (2000) Cell 103, 263-71.
- Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
- Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
- Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
- Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
- Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
- Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85.
- Dai, J. et al. (2005) Genes Dev 19, 472-88.
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- 7233 PathScan® Acetyl-Histone H2A Sandwich ELISA Kit
- 7222 PathScan® Acetyl-Histone H2B (Lys20) Sandwich ELISA Kit
- 2934 Histone H2B (53H3) Mouse mAb
- 9681 Acetylated-Lysine Mouse mAb (Ac-K-103)
- 9814 Acetylated-Lysine (Ac-K2-100) Rabbit mAb
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7004 TMB Substrate
- 7002 STOP Solution
For Research Use Only. Not For Use In Diagnostic Procedures.