Product Pathways - PathScan ELISA
PathScan® Total S6 Ribosomal Protein Sandwich ELISA Antibody Pair #7203
| Kit Includes | Volume | Cap Color |
|---|---|---|
| S6 Ribosomal Protein Capture Antibody (100X) | 0.4 milliliters | Pink |
| S6 Ribosomal Protein Detection Antibody (100X) | 0.4 milliliters | Blue |
| Anti-Rabbit IgG HRP-Linked Antibody (1000X) | 0.04 milliliters | Red |
Capture and Detection Antibodies are stored at 4°C. HRP-Linked Secondary Antibody is stored at -20°C.
Species Cross-Reactivity
H M
Reactivity Key:
H=Human
M=Mouse
Species cross-reactivity is determined by Western blot.
Description
CST's PathScan® Total S6 Ribosomal Protein Sandwich ELISA Antibody Pair is being offered as an alternative to our PathScan® Total S6 Ribosomal Protein Sandwich ELISA Kit #7225. Capture and Detection antibodies (100X stocks) and an HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are provided for performing 4 x 96 well ELISAs. The S6 Ribosomal Protein Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added, followed by S6 Ribosomal Protein Detection Antibody and HRP-conjugated secondary antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance at 450 nm is proportional to the quantity of Total S6 Ribosomal protein.*Antibodies in this kit are custom formulations specific to the kit.
Sandwich ELISA
The relationship between lysate protein concentration from untreated and PDGF treated NIH/3T3 cells and the absorbance at 450 nm using PathScan® Total S6 Ribosomal Protein Sandwich ELISA Antibody Pair #7203 is shown. NIH/3T3 cells were starved overnight, treated with PDGF for 20 minutes at 37°C and then lysed.
Background
One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240 and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).
- Dufner, A. and Thomas, G. (1999) Exp. Cell Res. 253, 100-109.
- Peterson, R.T. and Schreiber, S.L. (1998) Curr. Biol. 8, R248-R250.
- Jefferies, H.B. et al. (1997) EMBO J. 16, 3693-3704.
- Ferrari, S. et al. (1991) J. Biol. Chem. 266, 22770-22775.
- Flotow, H. and Thomas, G. (1992) J. Biol. Chem. 267, 3074-3078.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9808 Phosphate Buffered Saline (PBS-20X)
- 9809 Phosphate Buffered Saline with Tween 20 (PBST-20X)
- 9998 BSA
- 7004 TMB Substrate
- 7002 STOP Solution
- 7225 PathScan® Total S6 Ribosomal Protein Sandwich ELISA Kit
- 7205 PathScan® Phospho-S6 Ribosomal Protein (Ser235/236) Sandwich ELISA Kit
- 7201 PathScan® Phospho-S6 Ribosomal Protein (Ser235/236) Sandwich ELISA Antibody Pair
- 2217 S6 Ribosomal Protein (5G10) Rabbit mAb
- 2317 S6 Ribosomal Protein (54D2) Mouse mAb
- 7074 Anti-rabbit IgG, HRP-linked Antibody
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
