Product Pathways - PathScan ELISA
PathScan® Phospho-MEK1 (Ser217/221) Sandwich ELISA Antibody Pair #7211
|7211S||1 Kit (Reagents for 4 x 96 well plates)||---||In Stock||---|
|7211||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
|Kit Includes||Volume||Cap Color|
|Total MEK1 Capture Ab(100X)||0.4 ml||Pink|
|P-MEK1/2 (S217/221) Detection Ab (100X)||0.4 ml||Blue|
|Anti-rabbit IgG, HRP-linked Antibody (1000X)||0.04 ml||Red|
Storage: Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.
Important Ordering Details
Product is assembled upon order to ensure maximum activity. United States: Please allow up to two weeks for your order to be processed and shipped. Outside of the United States: Please allow up to three weeks, depending on the country, for your order to be processed and shipped.
CST's PathScan® Phospho-MEK1 (Ser217/221) Sandwich ELISA Antibody Pair is offered as an economical alternative to our PathScan® Phospho-MEK1 (Ser217/221) Sandwich ELISA Kit #7175. Capture and Detection antibodies (100X stocks) and HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The MEK1 Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added followed by Phospho-MEK1/2 (Ser217/221) Detection Antibody and anti-Rabbit IgG, HRP-conjugated antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of phospho-MEK1/2 (Ser217/221) protein.
Antibodies in kit are custom formulations specific to kit.
Specificity / Sensitivity
For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
The relationship between lysate protein concentration from untreated and PDGF-treated NIH/3T3 cells and the absorbance at 450 nm using PathScan® Phospho-MEK1 (Ser217/221) Sandwich ELISA Antibody Pair #7211 is shown. NIH/3T3 cells were treated with PDGF for 20 minutes at 37ºC and then lysed.
MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 9808 Phosphate Buffered Saline (PBS-20X)
- 9809 Phosphate Buffered Saline with Tween® 20 (PBST-20X)
- 9998 BSA
- 7004 TMB Substrate
- 7002 STOP Solution
- 7175 PathScan® Phospho-MEK1 (Ser217/221) Sandwich ELISA Kit
- 7165 PathScan® Total MEK1 Sandwich ELISA Kit
- 7215 PathScan® Total MEK1 Sandwich ELISA Antibody Pair
- 9154 Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb
- 9121 Phospho-MEK1/2 (Ser217/221) Antibody
- 2352 MEK1 (61B12) Mouse mAb
- 9122 MEK1/2 Antibody
- 9146 MEK1 (30C8) Rabbit mAb
- 9126 MEK1/2 (47E6) Rabbit mAb
- 4694 MEK1/2 (L38C12) Mouse mAb
- 9803 Cell Lysis Buffer (10X)
- 7074 Anti-rabbit IgG, HRP-linked Antibody
For Research Use Only. Not For Use In Diagnostic Procedures.
PathScan® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.