Product Pathways - PathScan ELISA
PathScan® Total MEK1 Sandwich ELISA Antibody Pair #7215
|Kit Includes||Volume||Cap Color|
|Total MEK1 Capture Ab(100X)||0.4 ml||Pink|
|Total MEK1/2 Detection Ab (100X)||0.4 ml||Blue|
|Anti-rabbit IgG, HRP-linked Antibody||0.04 ml||Red|
Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Important Ordering Details
Product is assembled upon order to ensure maximum activity. Domestic: Please allow up to two weeks for your order to be processed and shipped. International: Please allow up to three weeks, depending on the country, for your order to be processed and shipped.
CST's PathScan® Total MEK1 Sandwich ELISA Antibody Pair is offered as an economical alternative to our PathScan® Total MEK1 Sandwich ELISA Kit #7165. Capture and Detection antibodies (100X stocks) and HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The MEK1 Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added followed by a MEK1/2 Detection Antibody and anti-Rabbit IgG, HRP-conjugated antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of total MEK1/2 protein.Antibodies in kit are custom formulations specific to kit.
Specificity / Sensitivity
For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.
- Crews, C.M. et al. (1992) Science 258, 478-480.
- Alessi, D.R. et al. (1994) EMBO J. 13, 1610-1619.
- Rosen, L.B. et al. (1994) Neuron 12, 1207-1221.
- Cowley, S. et al. (1994) Cell 77, 841-852.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 9808 Phosphate Buffered Saline (PBS-20X)
- 9809 Phosphate Buffered Saline with Tween 20 (PBST-20X)
- 9998 BSA
- 7004 TMB Substrate
- 7002 STOP Solution
- 7165 PathScan® Total MEK1 Sandwich ELISA Kit
- 7175 PathScan® Phospho-MEK1 (Ser217/221) Sandwich ELISA Kit
- 7211 PathScan® Phospho-MEK1 (Ser217/221) Sandwich ELISA Antibody Pair
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 2352 MEK1 (61B12) Mouse mAb
- 9122 MEK1/2 Antibody
- 9146 MEK1 (30C8) Rabbit mAb
- 9126 MEK1/2 (47E6) Rabbit mAb
- 4694 MEK1/2 (L38C12) Mouse mAb
- 9803 Cell Lysis Buffer (10X)
For Research Use Only. Not For Use In Diagnostic Procedures.