Product Pathways - PathScan ELISA
PathScan® Phospho-4E-BP1 (Thr37/Thr46) Sandwich ELISA Kit #7216
| Kit Includes | Volume | Solution Color |
|---|---|---|
| 4E-BP1 (Thr37/46) Ab Coated Microwells | 96 tests | |
| 4E-BP1 Detection Ab | 11 milliliters | Green |
| Anti-Mouse IgG HRP-Linked Ab | 11 milliliters | Red |
| TMB Substrate | 11 milliliters | Colorless |
| STOP Solution | 11 milliliters | Colorless |
| Sealing Tape | 2 sheets | |
| 20X Wash Buffer | 25 milliliters | Colorless |
| Sample Diluent | 25 milliliters | Blue |
| Cell Lysis Buffer (10X) # 9803 | 15 milliliters | Yellowish |
Note: 12 8-well modules –Each module is designed to break apart for 8 tests.
Note: Kit should be stored at 4°C with the exception of Cell Lysis Buffer (10X), which is stored at –20°C (packaged separately).
Species Cross-Reactivity
H
Reactivity Key: H=Human
Description
CST's PathScan® Phospho-4E-BP1 (Thr37/46) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of 4E-BP1 when phosphorylated at Thr37/46. A Phospho-4E-BP1 (Thr37/46) Rabbit Antibody* has been coated onto the microwells. After incubation with cell lysates, phospho-4E-BP1 (Thr37/46) is captured by the coated antibody. Following extensive washing, a 4E-BP1 Mouse Detection Antibody* is added to detect the captured phospho-4E-BP1 protein. Anti-mouse IgG, HRP-linked Antibody #7076* is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of absorbance for this developed color is proportional to the quantity of 4E-BP1 phosphorylated at Thr37/46.* Antibodies in kit are custom formulations specific to kit.
Specificity / Sensitivity
CST's PathScan® Phospho-4E-BP1 (Thr37/46) Sandwich ELISA Kit detects endogenous levels of phospho-4E-BP1 when phosphoryated at Thr37/46. As shown in Figure 1, using the Phospho-4E-BP1 (Thr37/46) ELISA Kit #7216, a significant induction of 4E-BP1 phosphorylation at Thr37/46 is detected in serum and amino acid starved HEK-293T cells treated with insulin for 30 minutes after replenishing the amino acids.
Sandwich ELISA
Figure 2: The relationship between the protein concentration of lysate from amino acid (AA)/untreated and AA/insulin-treated HEK-293T and the absorbance at 450 nM is shown.
Sandwich ELISA
Figure 1: Treatment of HEK-293T cells with amino acids and insulin stimulates phosphorylation of 4E-BP1 at Thr37/46, as detected by PathScan® Phospho-4E-BP1 (Thr37/46) Sandwich ELISA Kit #7216, but does not affect the level of total 4E-BP1 protein detected by Western analysis. HEK-293T cells (70-80% confluent) were starved overnight and deprived of amino acids for 1 hour. The amino acids were replenished for 1 hour. Cells were either untreated or stimulated with 100 nM insulin for 30 minutes at 37°C. λ phosphatase treatement of control cell lysates (4000 U/mL for 60 minutes at 37°C) abolishes the basal phosphorylation of 4E-BP1 as shown by both sandwich ELISA and Western analysis. The absorbance readings at 450 nM are shown in the top figure, while the corresponding Western blots, using 4E-BP1 Antibody #9452 (left panel) or Phospho-4E-BP1 (Thr37/46) Antibody #9459 (right panel), are shown in the bottom figure.
Background
Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the eIF4E translation initiation factor. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR on Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).
- Pause, A. et al. (1994) Nature 371, 762-767.
- Brunn, G.J. et al. (1997) Science 277, 99-101.
- Gingras, A.C. et al. (1998) Genes Dev. 12, 502-513.
- Fadden, P. et al. (1997) J. Biol. Chem. 272, 10240-10247.
- Gingras, A.C. et al. (1999) Genes Dev. 13, 1422-1437.
Application References
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Companion Products
- 9459 Phospho-4E-BP1 (Thr37/46) Antibody
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 9644 4E-BP1 (53H11) Rabbit mAb
- 9452 4E-BP1 Antibody
- 9456 Phospho-4E-BP1 (Ser65) (174A9) Rabbit mAb
- 9451 Phospho-4E-BP1 (Ser65) Antibody
- 2855 Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb
- 9455 Phospho-4E-BP1 (Thr70) Antibody
- 4923 Nonphospho-4E-BP1 (Thr46) (87D12) Rabbit mAb
- 2845 4E-BP2 Antibody
- 9803 Cell Lysis Buffer (10X)
