Cell Signaling Technology

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PathScan® Phospho-PDGF Receptor α/β (panTyr) Sandwich ELISA Kit #7235

Kit Includes Volume Solution Color
PDGF Receptor alpha/beta Rabbit Antibody Coated Microwells 96 tests
Biotinylated Phospho-Tyrosine Mouse Detection Antibody 11 milliliters Green
HRP-linked Streptavidin 11 milliliters Red
TMB Substrate 11 milliliters Colorless
STOP Solution 11 milliliters Colorless
Sealing Tape 2 sheets
20X Wash Buffer 25 milliliters Colorless
Sample Diluent 25 milliliters Blue
Cell Lysis Buffer (10X) # 9803

Note: 12 8-well modules –Each module is designed to break apart for 8 tests.
Note: Kit should be stored at 4°C with the exception of Cell Lysis Buffer (10X), which is stored at –20°C (packaged separately).

Species Cross-Reactivity

H M

Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by Western blot.

Description

The PathScan® Phospho-PDGF Receptor α/β (panTyr) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of PDGF receptor α/β when tyrosine phosphorylated. A PDGF Receptor α/β Rabbit Antibody* has been coated onto the microwells. After incubation with cell lysates, PDGF Receptor α/β (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, a Biotinylated Phospho-Tyrosine Mouse Detection Antibody* is added to detect tyrosine phosphorylation of the captured PDGF receptor α/β protein. HRP-linked Strepavidin* is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of PDGF receptor α/β phosphorylated on tyrosine.* Antibodies in kit are custom formulations specific to kit.The PathScan® Phospho-c-Kit (panTyr) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of c-Kit when tyrosine phosphorylated. A c-Kit Mouse mAb* has been coated onto the microwells. After incubation with cell lysates, c-Kit (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, a Biotinylated Phospho-Tyrosine Mouse Detection Antibody* is added to detect tyrosine phosphorylation of the captured c-Kit protein. HRP-linked Strepavidin* is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of c-Kit phosphorylated on tyrosine.* Antibodies in kit are custom formulations specific to kit.

Specificity / Sensitivity

CST's PathScan® Phospho-PDGF Receptor α/β (panTyr) Sandwich ELISA Kit #7235 detects PDGF receptor α/β when tyrosine phosphorylated. As shown in Figure 1, a significant induction of PDGF Receptor α/β tyrosine phosphorylation can be detected in MG63 cells following treatment with PDGF using the Phospho-PDGF Receptor α/β (panTyr) Sandwich ELISA Kit #7235. The level of total PDGF receptor α (phospho and nonphospho) remains unchanged as shown by Western analysis and by PathScan® Total PDGF Receptor α Sandwich ELISA Kit #7318. Western blot analysis of protein captured in the PDGF receptor α/β antibody coated microwell indicates that PDGF receptor α/β (phospho and nonphospho) has been captured (data not shown). The Western blot also shows a major band corresponding to the phospho-PDGF receptor α/β protein when biotinylated anti-tyrosine antibody is used as probe (see Figure 3).

Sandwich ELISA

Sandwich ELISA

Figure 1. Treatment of MG63 cells with PDGF stimulates tyrosine phosphorylation of PDGF Receptor α/β, detected by PathScan® Phospho-PDGF Receptor α/β (panTyr) Sandwich ELISA Kit #7235, but does not affect the level of total PDGF Receptor α or β protein detected by either PathScan® Total PDGF Receptor α Sandwich ELISA Kit #7318 or Western analysis. The absorbance readings at 450 nm are shown in the top figure, while the corresponding Western blot using PDGF Receptor α Rabbit mAb #3174 (panel A), PDGF Receptor β Rabbit mAb #3169 (panel B), Phospho-PDGF Receptor α (Tyr754) (23B2) Rabbit mAb #2992 (panel C) or Phospho-PDGF Receptor β (Tyr771) (76D6) Rabbit mAb #3173 (panel D) is shown in the bottom figure.

Sandwich ELISA

Sandwich ELISA

Figure 2. The relationship between protein concentration of untreated or PDGF-treated MG63 cell lysates and the absorbance at 450 nm is shown. Cells were serum starved overnight and then treated with PDGF (50 ng/ml) for 7 min. at 37oC.

Sandwich ELISA

Sandwich ELISA

Figure 3. Kit specificity as demonstrated by Western analysis of the ELISA microwell captured protein. Lysates were prepared from MG63 cells and incubated in microwells coated with the PDGF Receptor α/β capture antibody. Wells were washed, and the captured protein was solubilized in SDS gel loading buffer. Western analysis of MG63 cell starting lysate (lanes 1 & 2) and the captured protein (lanes 3 & 4) was performed using Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Biotinylated) #9417.The major band detected in the captured material corresponds to the phospho-PDGF Receptor α/β protein (lanes 3 & 4).


Background

The proteins of the platelet derived growth factor (PDGF) family exist as several disulphide-bonded, dimeric isoforms (PDGF AA, PDGF AB, PDGF BB, PDGF CC and PDGF DD) that bind in a specific pattern to two closely related receptor tyrosine kinases, PDGF receptor α (PDGFRα) and PDGF receptor β (PDGFRβ). PDGFRα and PDGFRβ share 75% to 85% sequence homology between their two intracellular kinase domains while the kinase insert and carboxy-terminal tail regions display a lower level (27% to 28%) of homology (1). PDGF Receptor α homodimers bind all PDGF isoforms except those containing PDGF D. PDGF Receptor β homodimers bind PDGF BB and DD isoforms, as well as the PDGF AB heterodimer. The heteromeric PDGF α/β receptor binds PDGF B, C, and D homodimers as well as the PDGF AB heterodimer (2). PDGFRα and PDGFRβ can each form heterodimers with EGFR, which is also activated by PDGF (3). Various cells differ in the total number of receptors present and in the receptor subunit composition, which may account for responsive differences among cell types to PDGF binding (4). Ligand binding induces receptor dimerization and autophosphorylation, followed by binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules such as Grb2, Src, GAP, PI3 kinase, PLCγ and Nck. A number of different signaling pathways are initiated by activated PDGF receptors and lead to control of cell growth, actin reorganization, migration and differentiation (5). Tyr751 in the kinase-insert region of PDGFRβ is the docking site for PI3 kinase (6). Phosphorylated pentapeptides derived from Tyr751 of PDGFRβ (pTyr751-Val-Pro-Met-Leu) inhibit the association of the carboxy-terminal SH2 domain of the p85 subunit of PI3 kinase with PDGFRβ (7). Tyr740 is also required for PDGFRβ mediated PI3 kinase activation (8).

  1. Deuel, T.F. et al. (1988) Biofactors 1, 213-217.
  2. Bergsten, E. et al. (2001) Nat. Cell Biol. 3, 512-516.
  3. Betsholtz, C. et al. (2001) Bioessays 23, 494-507.
  4. Coughlin, S.R. et al. (1988) Prog. Clin. Biol. Res. 266, 39-45.
  5. Ostman, A. and Heldin, C.H. (2001) Adv. Cancer Res. 80, 1-38.
  6. Panayotou, G. et al. (1992) EMBO J. 11, 4261-4272.
  7. Ramalingam, K. et al. (1995) Bioorg. Med. Chem. 3, 1263-1272.
  8. Kashishian, A. et al. (1992) EMBO J. 11, 1373-1382.

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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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