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XML generation date: 2026-06-05 17:28:19.105
Product last modified at: 2025-11-14T08:04:44.763Z
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PDP - Template Name: ELISA Kit
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  1. Products /
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  3. PathScan® Phospho-IRS-1 (Ser302) Sandwich ELISA Kit

PathScan® Phospho-IRS-1 (Ser302) Sandwich ELISA Kit #7283

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Inquiry Info. # 7283

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    Product Specifications

    REACTIVITY H
    Application Key:
    • ELISA+-ELISA and/or ELISA-like Assays 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    The PathScan® Phospho-IRS-1 (Ser302) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of IRS-1 when phosphorylated at Ser302. An IRS-1 Mouse Antibody* has been coated onto the microwells. After incubation with cell lysates, IRS-1 (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, a Phospho-IRS-1 (Ser302) Rabbit Detection Antibody* is added to detect phosphorylation of Ser302 on the captured IRS-1 protein. Anti-rabbit IgG, HRP-linked Antibody #7074* is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of IRS-1 phosphorylated at Ser302.
    * Antibodies in kit are custom formulations specific to kit.

    Protocol

    Specificity / Sensitivity

    PathScan® Phospho-IRS-1 (Ser302) Sandwich ELISA Kit #7283 detects endogenous levels of IRS-1 when phosphorylated at Ser302. As shown in Figure 1, a significant induction of IRS-1 phosphorylation at Ser302 can be detected in hSkMC cells following treatment with insulin using this Sandwich ELISA Kit #7283. The level of total IRS-1 (phospho and nonphospho) remains unchanged as shown by Western analysis and by PathScan® Total IRS-1 Sandwich ELISA Kit #7328 (Figure 1). This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

    Species Reactivity:

    Human

    Background

    Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6) while Ser789 is phosphorylated by SIK-2, a member of the AMPK family (7). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively (8,9). Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (10).

    Alternate Names

    HIRS-1; Insulin receptor substrate 1; IRS-1; IRS1

    Database Links

    UniProt ID: P35568


    Entrez-Gene Id: 3667


    Limited Uses

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    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    PathScan is a registered trademark of Cell Signaling Technology, Inc.
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