Product Pathways - PathScan ELISA
PathScan® Total β-Catenin Sandwich ELISA Antibody Pair #7309
| Kit Includes | Volume | Cap Color |
|---|---|---|
| Beta-Catenin Rabbit Capture Antibody (100X) | 0.4 milliliters | Pink |
| Beta-Catenin Mouse Detection Antibody (100X) | 0.4 milliliters | Blue |
| Anti-Mouse IgG, HRP-Linked Antibody (1000X) | 0.04 milliliters | Yellow |
Capture and Detection Antibodies are stored at 4°C. HRP-Linked Secondary Antibody is stored at -20°C.
Species Cross-Reactivity
H M R
Reactivity Key: H=Human M=Mouse R=Rat
Description
CST's PathScan® Total β-Catenin Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Total β-Catenin Sandwich ELISA Kit #7308. Capture and Detection antibodies (100X stocks) and HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The β-Catenin Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added followed by a β-Catenin Detection Antibody and anti-Mouse IgG, HRP conjugated antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of total β-Catenin protein.*Antibodies in this kit are custom formulations specific to the kit.
Sandwich ELISA
The relationship between protein concentration of lysates from untreated and Calyculin A-treated 293 cells and the absorbance at 450 nm using PathScan® Total β-Catenin Sandwich ELISA Antibody Pair #7309 is shown. 293 cells were treated with Calyculin A #9902 (50 nM) for 10 min at 37?C, and then lysed.
Background
β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin on Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3 (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37 and Thr41 (7). Mutations in these phosphorylation sites, which result in the stabilization of β-catenin protein levels, have been found in many tumor cell lines (8).
- Cadigan, K.M. and Nusse, R. (1997) Genes Dev. 11, 3286-3305.
- Wodarz, A. and Nusse, R. (1998) Annu. Rev. Cell. Dev. Biol. 14, 59-88.
- Polakis, P. (1999) Curr. Opin. Genet. Dev. 9, 15-21.
- Amit, S. et al. (2002) Genes Dev. 16, 1066-1076.
- Lin, C. et al. (2002) Cell 108, 837-847.
- Yanagawa, S. et al. (2002) EMBO J. 21, 1733-1742.
- Yost, C. et al. (1996) Genes Dev. 10, 1443-1454.
- Morin, P.J. (1997) Science 275, 1787-1790.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9808 Phosphate Buffered Saline (PBS-20X)
- 9809 Phosphate Buffered Saline with Tween 20 (PBST-20X)
- 9998 BSA
- 7004 TMB Substrate
- 7002 STOP Solution
- 9803 Cell Lysis Buffer (10X)
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7308 PathScan® Total β-Catenin Sandwich ELISA Kit
- 9562 β-Catenin Antibody
- 9582 β-Catenin (6B3) Rabbit mAb
- 2698 β-Catenin (L87A12) Mouse mAb
- 9581 β-Catenin Antibody (Amino-terminal Antigen)
- 9587 β-Catenin Antibody (Carboxy-terminal Antigen)
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.