Product Pathways - PathScan ELISA
PathScan® Total c-Jun Sandwich ELISA Antibody Pair #7314
| Kit Includes | Volume | Cap Color |
|---|---|---|
| Total c-Jun Capture Antibody (100X) | 0.4 milliliters | Pink |
| Total c-Jun Detection Antibody (100X) | 0.4 milliliters | Blue |
| Anti-Mouse IgG HRP-Linked Antibody (1000X | 0.04 milliliters | Yellow |
Capture and Detection Antibodies are stored at 4°C. HRP-Linked Secondary Antibody is stored at -20°C.
Species Cross-Reactivity
H M R
Reactivity Key: H=Human M=Mouse R=Rat
Description
CST's PathScan® Total c-Jun Sandwich ELISA Antibody Pair is being offered as an alternative to our PathScan® Total c-Jun Sandwich ELISA Kit II #7150. Capture and Detection antibodies (100X stocks) and a HRP-Conjugated Secondary Antibody (1000X stock) is supplied. Sufficient reagents are provided for performing 4 x 96 well ELISAs. The c-Jun Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added, followed by c-Jun Detection Antibody and HRP-Conjugated Secondary Antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance at 450 nm is proportional to the quantity of total c-Jun protein.*Antibodies in this kit are custom formulations specific to the kit.
Background
c-Jun is a component of the transcription factor AP-1, which binds and activates transcription at TRE/AP-1 elements. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 (1,2). Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun-dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress-induced signaling pathways. The MAP kinase homologue SAPK/JNK binds to the amino-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun (3,4).
- Binetruy, B. et al. (1991) Nature 351, 122-127.
- Smeal, T. et al. (1991) Nature 354, 494-496.
- Derijard, B. et al. (1994) Cell 76, 1025-1037.
- Kyriakis, J.M. et al. (1994) Nature 369, 156-160.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9808 Phosphate Buffered Saline (PBS-20X)
- 9809 Phosphate Buffered Saline with Tween 20 (PBST-20X)
- 9998 BSA
- 7004 TMB Substrate
- 7002 STOP Solution
- 7150 PathScan® Total c-Jun Sandwich ELISA Kit II
- 7145 PathScan® Phospho-c-Jun (Ser63) Sandwich ELISA Kit II
- 9165 c-Jun (60A8) Rabbit mAb
- 2315 c-Jun (L70B11) Mouse mAb