Product Pathways - PathScan ELISA
PathScan® Total S6 Ribosomal Protein Chemiluminescent Sandwich ELISA Kit #7337
When ordering five or more kits, please contact us for processing time and pricing at firstname.lastname@example.org.
|Kit Includes||Volume||Solution Color|
|S6 Ribosomal Protein Mouse Ab Coated Microwells||96 tests|
|S6 Ribosomal Protein Rabbit Detection Antibody||5.5 ml||Green|
|Anti-rabbit IgG, HRP-Linked Ab||5.5 ml||Red|
|Luminol/Enhancer Solution||3 ml||Colorless|
|Stable Peroxide Buffer||3 ml||Colorless|
|Sealing Tape||2 sheets|
|ELISA Wash Buffer (20X)||25 ml||Colorless|
|ELISA Sample Diluent||25 ml||Blue|
|Cell Lysis Buffer (10X) #9803||15 ml||Yellowish|
Note: 12 8-well modules –Each module is designed to break apart for 8 tests.
Note: Kit should be stored at 4°C with the exception of Cell Lysis Buffer, which is stored at –20°C (packaged separately).
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
The PathScan® Total S6 Ribosomal Protein Chemiluminescent Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of total S6 ribosomal protein with a chemiluminescent readout. Chemiluminescence ELISAs often have a wider dynamic range and higher sensitivity than conventional chromogenic detection. This chemiluminescent ELISA, which is offered in low volume microplates, shows increased signal and sensitivity while using smaller sample size. A S6 Ribosomal Protein Mouse mAb has been coated on the microwells. After incubation with cell lysates, the S6 ribosomal protein is captured by the coated antibody. Following extensive washing, S6 Ribosomal Protein Rabbit Antibody is added to detect the captured total S6 ribosomal protein. Anti-rabbit IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. Chemiluminescent reagent is added for signal development. The magnitude of light emission, measured in relative light units (RLU), is proportional to the quantity of totalS6 ribosomal protein.Antibodies in kit are custom formulations specific to kit.
Specificity / Sensitivity
PathScan® Total S6 Ribosomal Protein Chemiluminescent Sandwich ELISA Kit #7337 detects endogenous levels of total S6 ribosomal protein in human and mouse cells. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Figure 1. Relationship between protein concentration from nonphospho cell lysate and PDGF-treated NIH/3T3 cell lysate and immediate light generation with chemiluminescent substrate is shown. NIH/3T3 cells (80% confluence) were lysed without the addition of phosphatase inhibitors to the lysis buffer (nonphospho lysate) or treated with hPDGF-BB #8912 (50 ng/ml, 30 min at 37ºC) and lysed with Cell Lysis Buffer (10X) #9803. Graph inset corresponding to the shaded area shows high sensitivity and a linear response at the low protein concentration range.
One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).
- Dufner, A. and Thomas, G. (1999) Exp. Cell Res. 253, 100-109.
- Peterson, R.T. and Schreiber, S.L. (1998) Curr. Biol. 8, R248-R250.
- Jefferies, H.B. et al. (1997) EMBO J. 16, 3693-3704.
- Ferrari, S. et al. (1991) J. Biol. Chem. 266, 22770-22775.
- Flotow, H. and Thomas, G. (1992) J. Biol. Chem. 267, 3074-3078.
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* Product-specific protocol.
- 7225 PathScan® Total S6 Ribosomal Protein Sandwich ELISA Kit
- 7205 PathScan® Phospho-S6 Ribosomal Protein (Ser235/236) Sandwich ELISA Kit
- 7316 PathScan® Phospho-S6 Ribosomal Protein (Ser235/236) Chemiluminescent Sandwich ELISA Kit
- 7203 PathScan® Total S6 Ribosomal Protein Sandwich ELISA Antibody Pair
- 7201 PathScan® Phospho-S6 Ribosomal Protein (Ser235/236) Sandwich ELISA Antibody Pair
- 2217 S6 Ribosomal Protein (5G10) Rabbit mAb
- 2317 S6 Ribosomal Protein (54D2) Mouse mAb
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 9803 Cell Lysis Buffer (10X)
For Research Use Only. Not For Use In Diagnostic Procedures.