Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Nuclear Receptor Signaling

Androgen Receptor (D6F11) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #7397

Applications Reactivity Sensitivity Isotype
IF-IC F H Endogenous Rabbit IgG

Applications Key:  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Androgen Receptor (D6F11) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of total androgen receptor protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein corresponding to residues near the amino terminal region of human androgen receptor protein.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat (blue) and LNCaP (green) cells using Androgen Receptor (D6F11) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate).

IF-IC

IF-IC

Confocal immunofluorescent analysis of LNCaP (positive, left) and DU 145 (negative, right) cells using Androgen Receptor (D6F11) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor). Actin filaments were labeled with Alexa Fluor® 488 phalloidin (green).

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometric and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Androgen Receptor (D6F11) XP® Rabbit mAb #5153.

Background

Androgen receptor (AR), a zinc finger transcription factor belonging to the nuclear receptor superfamily, is activated by phosphorylation and dimerization upon ligand binding (1). This promotes nuclear localization and binding of AR to androgen response elements in androgen target genes. AR plays a crucial role in several stages of male development and the progression of prostate cancer (2,3).

  1. Li, J. and Al-Azzawi, F. (2009) Maturitas 63, 142-8.
  2. Avila, D.M. et al. (2001) J. Steroid. Biochem. Mol. Biol. 76, 135-142.
  3. Montgomery, J.S. et al. (2001) J. Pathol. 195, 138-146.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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