Cell Signaling Technology

Cellular Dyes

DRAQ7™ #7406

Protocols

Directions for Use

Flow Cytometry: Prepare cells for DRAQ7™ staining by resuspending cells in PBS at a concentration of no more than 5 x 105 cells/ml. For each 0.5 ml of cell suspension, dilute DRAQ7™ 1:100 by adding 5 μl to suspended cells, achieving a final concetration of 3 μM. Gently mix and incubate for 10 minutes on ice. Cells should be analyzed directly in the presence of DRAQ7™.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of live Jurkat cells treated with Staurosporine #9953. Gated population represents DRAQ7™-positive apoptotic cells.

Spectra

Spectra

DRAQ7® emission spectra when excited at various wavelengths.

Background

DRAQ7™ is a far-red fluorescent DNA dye that only stains the nuclei in dead and permeabilized cells. It can be used in combination with GFP, FITC, and PE labels.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


For Research Use Only. Not For Use In Diagnostic Procedures.

Products