Product Pathways - Tyrosine Kinase / Adaptors
14-3-3 ζ/δ (D7H5) Rabbit mAb #7413
|7413S||100 µl (10 western blots)||---||In Stock||---|
|7413||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
|W||1:1000||Human, Mouse, Rat, Monkey, Pig||Endogenous||28||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Chicken.
Specificity / Sensitivity
14-3-3 ζ/δ (D7H5) Rabbit mAb recognizes endogenous levels of total 14-3-3 ζ/δ protein. Although this antibody demonstrates a strong preference for 14-3-3 ζ/δ, it will also detect purified, recombinant 14-3-3 α/β. It does not cross-react with any other known mammalian 14-3-3 isoforms.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg80 of human 14-3-3 ζ/δ protein.
The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways (1,2). 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ, and η that have been identified in mammals. The initially described α and δ isoforms are confirmed to be phosphorylated forms of β and ζ, respectively (3). Through their amino-terminal α helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases, and other signaling molecules (3,4). The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation independent interactions has been observed (4). 14-3-3 binding masks specific sequences of the target protein, and therefore, modulates target protein localization, phosphorylation state, stability, and molecular interactions (1-4). 14-3-3 proteins may also induce target protein conformational changes that modify target protein function (4,5). Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities (4). Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes (2,3).
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 8312 14-3-3 (pan) Antibody
- 5521 14-3-3 η (D23B7) Rabbit mAb
- 5522 14-3-3 γ (D15B7) Rabbit mAb
- 9635 14-3-3 ε Antibody
- 9636 14-3-3 β/α Antibody
- 9638 14-3-3 τ Antibody
- 9640 14-3-3 η Antibody
- 9601 Phospho-(Ser) 14-3-3 Binding Motif Antibody
- 9606 Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb
- 2981 Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.