Cell Signaling Technology

Product Pathways - MAPK Signaling

MAPKAPK-5 (D70A10) Rabbit mAb #7419

Applications Reactivity Sensitivity MW (kDa) Isotype
W H R Mk Endogenous 58 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MAPKAPK-5 (D70A10) Rabbit mAb recognizes endogenous levels of total MAPKAPK-5 protein. This antibody does not cross-react with MAPKAPK-2 or MAPKAPK-3.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly367 of human MAPKAPK-5 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MAPKAPK-5 (D70A10) Rabbit mAb.

Background

MAPKAPK-5 belongs to the mitogen-activated protein kinase (MAPK) activated protein kinases (MK) subfamily that includes MAPKAPK-2/MK2 and MK3/3pK. The MK subfamily is part of a family of protein kinase subfamilies downstream of MAPK pathways and includes ribosomal S6 kinase (RSKs), mitogen and stress activated kinases (MSKs) and MAPK-interacting kinases (MNKs). All MKs are activated by MAPK pathways and mediate important processes (e.g. gene expression, cell cycle progression) and have been implicated in inflammation and cancer (1,2). MAPKAPK-5 shows binding to and activation by p38 MAPK and extracellular-regulated kinases (Erk) (3,4). MAPKAPK-5 was shown to be activated by Erk3 and act as a chaperone to Erk3 (5,6). While overexpressed MAPKAPK-5 shares similar substrates with MAPKAPK-2, such as HSP27 and glycogen synthase, recent work with MAPKAPK-5 knock-out mice indicates distinct substrates and functional properties (7).

  1. Roux, P.P. and Blenis, J. (2004) Microbio.l Mol. Biol. Rev. 68, 320-344.
  2. Gaestel, M. (2006) Nat. Rev. Mol. Cell Biol. 7, 120-130.
  3. New, L. et al. (1998) EMBO J. 17, 3372-3384.
  4. Ni, H. et al. (1998) Biochem. Biophys. Res. Commun. 243, 492-496.
  5. Schumacher, S. et al. (2004) EMBO J. 23, 4770-4779.
  6. Seternes, O.M. et al. (2004) EMBO J. 23, 4780-4791.
  7. Shi, Y. et al. (2003) Mol. Cell Biol. 23, 7732-7741.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products