Cell Signaling Technology

Product Pathways - MAPK Signaling

MAPKAPK-3 (D54E4) Rabbit mAb #7421

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Mk Pg Endogenous 42 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MAPKAPK-3 (D54E4) Rabbit mAb recognizes endogenous levels of total MAPKAPK-3 protein. This antibody does not cross-react with MAPKAPK-2 or MAPKAPK-5.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro34 of human MAPKAPK-3 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MAPKAPK-3 (D54E4) Rabbit mAb.

Background

MAPKAPK-3 has a single potential SH3-binding site in the proline-rich amino terminus, a putative ATP-binding site, 2 MAP kinase phosphorylation site motifs, and a putative nuclear localization signal. It shares 72% nucleotide and 75% amino acid identity with MAPKAPK-2 (1). MAPKAPK-3 has been shown to be activated by growth inducers and stress stimulation of cells. In vitro studies have demonstrated that Erk, p38 MAP kinase, and Jun amino-terminal kinase are able to phosphorylate and activate MAPKAPK-3, which suggested a role for this kinase as an integrative element of signaling in both mitogen and stress responses (2). MAPKAPK-3 was reported to interact with, phosphorylate, and repress the activity of E47, which is a basic helix-loop-helix transcription factor involved in the regulation of tissue-specific gene expression and cell differentiation (3). MAPKAPK-3 may also support luteal maturation through the phosphorylation and activation of the nuclear transcription factor CREB (4).

  1. Sithanandam, G. et al. (1996) Mol. Cell Biol. 16, 868-876.
  2. Ludwig, S. et al. (1996) Mol. Cell Biol. 16, 6687-6697.
  3. Neufeld, B. et al. (2000) J. Biol. Chem. 275, 20239-20242.
  4. Maizels, E.T. et al. (2001) Mol. Endocrinol. 15, 716-733.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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