Product Pathways - Glucose Metabolism
SGK2 Kinase #7438
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Description
Purified recombinant full length human SGK2 (Met1-Cys367) kinase, supplied as a GST fusion protein.
Source / Purification
The GST-Kinase fusion protein was produced using a baculovirus expression system with a construct expressing full length human SGK2 (Met1-Cys367) (GenBank Accession No. NM_170693) with an amino-terminal GST tag. The protein was purified by one-step affinity chromatography using glutathione-agarose.
Gel Staining
Figure 1. The purity of the GST-SGK2 fusion protein was analyzed using SDS/PAGE followed by Coomassie stain.
Kinase Assay - Radiometric
Figure 2. SGK2 kinase activity was measured in a radiometric assay using the following reaction conditions: 4 mM MOPS, pH 7.2, 2.5 mM β-glycerophosphate, 1 mM EGTA, 0.4 mM EDTA, 4 mM MgCl2, 0.05 mM DTT, 50 μM ATP, 0.6 μM BSA, Substrate: Akt/SGK substrate, 400 ng/μL and recombinant SGK2: variable.
Quality Control
The theoretical molecular weight of the GST-SGK2 fusion protein is 67 kDa. The purified kinase was quality controlled for purity using SDS-PAGE followed by Coomassie stain [Fig.1]. SGK2 kinase activity was determined using a radiometric assay [Fig.2].
Background
Serum and glucocorticoid-inducible kinase (SGK) is a serine/threonine kinase closely related to Akt (1). SGK is rapidly induced in response to a variety of stimuli, including serum, glucocorticoid, follicle stimulating hormone, osmotic shock and mineralocorticoids. SGK activation can be accomplished via HGF PI3K-dependent pathways and by integrin-mediated PI3K-independent pathways (2,3). Induction and activation of SGK has been implicated in activating the modulation of antiapoptotic and cell cycle regulation (4-6). SGK also plays an important role in activating certain potassium, sodium and chloride channels, suggesting its involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion (2). SGK is negatively regulated by ubiquitin modification and proteasome degradation (7).
- Webster, M.K. et al. (1993) Mol. Cell. Biol. 13, 2031-2040.
- Kobayashi, T. and Cohen, P. (1999) Biochem. J. 339, 319-328.
- Park, J. et al. (1999) EMBO J. 18, 3024-3033.
- Brunet, A. et al. (2001) Mol. Cell. Biol. 21, 952-965.
- Mikosz, C.A. et al. (2001) J. Biol. Chem. 276, 16649-16654.
- Hayashi, M. et al. (2001) J. Biol. Chem. 276, 8631-8634.
- Brickley, D.R. et al. (2002) J. Biol. Chem. 277, 43064-43070.
Application References
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Companion Products
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
