Cell Signaling Technology

Product Pathways - Metabolism

PGAM1 Antibody #7534

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 28 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PGAM1 Antibody recognizes endogenous levels of total PGAM1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro128 of human PGAM1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PGAM1 Antibody.

Background

Phosphoglycerate mutase (PGAM1) catalyzes the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis (1-5). Research studies have shown increased PGAM1 expression in cancer (1-4) and mental disease (5). Specifically, PGAM1 was shown to be phosphorylated at His11 by phosphoenolpyruvate (PEP) in PKM2-expressing cells, suggesting a possible regulatory role for PGAM1 in actively proliferating cells via an alternative glycolytic pathway (1).

  1. Vander Heiden, M.G. et al. (2010) Science 329, 1492-9.
  2. Jacobowitz, D.M. et al. (2008) Microvasc Res 76, 89-93.
  3. Ren, F. et al. (2010) Mol Cancer 9, 81.
  4. Evans, M.J. et al. (2005) Nat Biotechnol 23, 1303-7.
  5. Martins-de-Souza, D. et al. (2009) BMC Psychiatry 9, 17.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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